Document Detail

Engineering specific cross-links in nucleic acids using glycol linkers.
MedLine Citation:
PMID:  18428859     Owner:  NLM     Status:  MEDLINE    
One of the most convenient methods for generating oligonucleotides possessing intra- or interstrand cross-links is through incorporation of oligoethylene glycol bridges by solid-phase synthesis. The reagents are commercially available or can be synthesized in a few easy synthetic steps. Unlike many other DNA and RNA cross-links, aspects of the structural and thermodynamic impact of modifying nucleic acids with oligoethylene glycols have been studied. This unit covers protection, phosphitylation, and preparation of the glycol linker for oligonucleotide synthesis.
T O'Dea; L W McLaughlin
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Current protocols in nucleic acid chemistry / edited by Serge L. Beaucage ... [et al.]     Volume:  Chapter 5     ISSN:  1934-9289     ISO Abbreviation:  -     Publication Date:  2001 May 
Date Detail:
Created Date:  2008-04-22     Completed Date:  2008-05-08     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101287865     Medline TA:  Curr Protoc Nucleic Acid Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  Unit 5.3     Citation Subset:  IM    
Boston College, Chestnut Hill, Massachusetts, USA.
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MeSH Terms
Biochemistry / methods*
Cross-Linking Reagents / chemistry*
Ethylene Glycol / chemistry
Glycols / chemistry*
Nucleic Acids / chemistry*
Oligonucleotides / chemistry
Trityl Compounds
Reg. No./Substance:
0/Cross-Linking Reagents; 0/Glycols; 0/Nucleic Acids; 0/Oligonucleotides; 0/Phosphites; 0/Trityl Compounds; 107-21-1/Ethylene Glycol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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