| Engineering entropy-driven reactions and networks catalyzed by DNA. | |
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MedLine Citation:
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PMID: 18006742 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Artificial biochemical circuits are likely to play as large a role in biological engineering as electrical circuits have played in the engineering of electromechanical devices. Toward that end, nucleic acids provide a designable substrate for the regulation of biochemical reactions. However, it has been difficult to incorporate signal amplification components. We introduce a design strategy that allows a specified input oligonucleotide to catalyze the release of a specified output oligonucleotide, which in turn can serve as a catalyst for other reactions. This reaction, which is driven forward by the configurational entropy of the released molecule, provides an amplifying circuit element that is simple, fast, modular, composable, and robust. We have constructed and characterized several circuits that amplify nucleic acid signals, including a feedforward cascade with quadratic kinetics and a positive feedback circuit with exponential growth kinetics. |
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Authors:
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David Yu Zhang; Andrew J Turberfield; Bernard Yurke; Erik Winfree |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Science (New York, N.Y.) Volume: 318 ISSN: 1095-9203 ISO Abbreviation: Science Publication Date: 2007 Nov |
Date Detail:
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Created Date: 2007-11-16 Completed Date: 2007-12-06 Revised Date: 2010-01-21 |
Medline Journal Info:
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Nlm Unique ID: 0404511 Medline TA: Science Country: United States |
Other Details:
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Languages: eng Pagination: 1121-5 Citation Subset: IM |
Affiliation:
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Computation and Neural Systems, California Institute of Technology, MC 136-93, 1200 East California Boulevard, Pasadena, CA91125, USA. dzhang@dna.caltech.edu |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Catalysis Chemical Engineering Computers, Molecular* DNA / chemistry* Entropy Equipment Design Feedback, Physiological Mice Nanotechnology Nucleic Acid Hybridization Rabbits |
| Chemical | |
Reg. No./Substance:
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9007-49-2/DNA |
| Comments/Corrections | |
Comment In:
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Science. 2007 Nov 16;318(5853):1078-9
[PMID:
18006730
]
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Erratum In:
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Science. 2009 Dec 18;326(5960):1633 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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