| Engineering a cyanobacterial cell factory for production of lactic acid. | |
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MedLine Citation:
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PMID: 22865063 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Metabolic engineering of microorganisms has become a versatile tool to facilitate production of bulk chemicals, fuels, etc. Accordingly, CO(2) has been exploited via cyanobacterial metabolism as a sustainable carbon source of biofuel and bioplastic precursors. Here we extended these observations by showing that integration of an ldh gene from Bacillus subtilis (encoding an l-lactate dehydrogenase) into the genome of Synechocystis sp. strain PCC6803 leads to l-lactic acid production, a phenotype which is shown to be stable for prolonged batch culturing. Coexpression of a heterologous soluble transhydrogenase leads to an even higher lactate production rate and yield (lactic acid accumulating up to a several-millimolar concentration in the extracellular medium) than those for the single ldh mutant. The expression of a transhydrogenase alone, however, appears to be harmful to the cells, and a mutant carrying such a gene is rapidly outcompeted by a revertant(s) with a wild-type growth phenotype. Furthermore, our results indicate that the introduction of a lactate dehydrogenase rescues this phenotype by preventing the reversion. |
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Authors:
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S Andreas Angermayr; Michal Paszota; Klaas J Hellingwerf |
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Publication Detail:
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Type: Journal Article Date: 2012-08-03 |
Journal Detail:
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Title: Applied and environmental microbiology Volume: 78 ISSN: 1098-5336 ISO Abbreviation: Appl. Environ. Microbiol. Publication Date: 2012 Oct |
Date Detail:
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Created Date: 2012-09-10 Completed Date: 2013-01-23 Revised Date: 2013-05-20 |
Medline Journal Info:
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Nlm Unique ID: 7605801 Medline TA: Appl Environ Microbiol Country: United States |
Other Details:
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Languages: eng Pagination: 7098-106 Citation Subset: IM |
Affiliation:
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Swammerdam Institute for Life Sciences and Netherlands Institute for Systems Biology, University of Amsterdam, Amsterdam, The Netherlands. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Bacillus subtilis
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enzymology,
genetics Genomic Instability L-Lactate Dehydrogenase / genetics, metabolism Lactic Acid / metabolism* Metabolic Engineering* Microbial Viability NADP Transhydrogenases / genetics, metabolism Synechocystis / genetics*, metabolism* |
| Chemical | |
Reg. No./Substance:
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50-21-5/Lactic Acid; EC 1.1.1.27/L-Lactate Dehydrogenase; EC 1.6.1.-/NADP Transhydrogenases |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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