Document Detail


Engineering a cyanobacterial cell factory for production of lactic acid.
MedLine Citation:
PMID:  22865063     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Metabolic engineering of microorganisms has become a versatile tool to facilitate production of bulk chemicals, fuels, etc. Accordingly, CO(2) has been exploited via cyanobacterial metabolism as a sustainable carbon source of biofuel and bioplastic precursors. Here we extended these observations by showing that integration of an ldh gene from Bacillus subtilis (encoding an l-lactate dehydrogenase) into the genome of Synechocystis sp. strain PCC6803 leads to l-lactic acid production, a phenotype which is shown to be stable for prolonged batch culturing. Coexpression of a heterologous soluble transhydrogenase leads to an even higher lactate production rate and yield (lactic acid accumulating up to a several-millimolar concentration in the extracellular medium) than those for the single ldh mutant. The expression of a transhydrogenase alone, however, appears to be harmful to the cells, and a mutant carrying such a gene is rapidly outcompeted by a revertant(s) with a wild-type growth phenotype. Furthermore, our results indicate that the introduction of a lactate dehydrogenase rescues this phenotype by preventing the reversion.
Authors:
S Andreas Angermayr; Michal Paszota; Klaas J Hellingwerf
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Publication Detail:
Type:  Journal Article     Date:  2012-08-03
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  78     ISSN:  1098-5336     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-09-10     Completed Date:  2013-01-23     Revised Date:  2013-07-12    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  7098-106     Citation Subset:  IM    
Affiliation:
Swammerdam Institute for Life Sciences and Netherlands Institute for Systems Biology, University of Amsterdam, Amsterdam, The Netherlands.
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MeSH Terms
Descriptor/Qualifier:
Bacillus subtilis / enzymology,  genetics
Genomic Instability
L-Lactate Dehydrogenase / genetics,  metabolism
Lactic Acid / metabolism*
Metabolic Engineering*
Microbial Viability
NADP Transhydrogenases / genetics,  metabolism
Synechocystis / genetics*,  metabolism*
Chemical
Reg. No./Substance:
50-21-5/Lactic Acid; EC 1.1.1.27/L-Lactate Dehydrogenase; EC 1.6.1.-/NADP Transhydrogenases
Comments/Corrections

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