Document Detail

Engineering cell physiology to enhance recombinant protein production in Escherichia coli.
MedLine Citation:
PMID:  17571257     Owner:  NLM     Status:  MEDLINE    
The advent of recombinant DNA technology has revolutionized the strategies for protein production. Due to the well-characterized genome and a variety of mature tools available for genetic manipulation, Escherichia coli is still the most common workhorse for recombinant protein production. However, the culture for industrial applications often presents E. coli cells with a growth condition that is significantly different from their natural inhabiting environment in the gastrointestinal tract, resulting in deterioration in cell physiology and limitation in cell's productivity. It has been recognized that innovative design of genetically engineered strains can highly increase the bioprocess yield with minimum investment on the capital and operating costs. Nevertheless, most of these genetic manipulations, by which traits are implanted into the workhorse through recombinant DNA technology, for enhancing recombinant protein productivity often translate into the challenges that deteriorate cell physiology or even jeopardize cell survival. An in-depth understanding of these challenges and their corresponding cellular response at the molecular level becomes crucial for developing superior strains that are more physiologically adaptive to the production environment to improve culture productivity. With the accumulated knowledge in cell physiology, whose importance to gene overexpression was to some extent undervalued previously, this review is intended to focus on the recent biotechnological advancement in engineering cell physiology to enhance recombinant protein production in E. coli.
C Perry Chou
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Publication Detail:
Type:  Journal Article; Review     Date:  2007-06-15
Journal Detail:
Title:  Applied microbiology and biotechnology     Volume:  76     ISSN:  0175-7598     ISO Abbreviation:  Appl. Microbiol. Biotechnol.     Publication Date:  2007 Sep 
Date Detail:
Created Date:  2007-08-20     Completed Date:  2007-12-06     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8406612     Medline TA:  Appl Microbiol Biotechnol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  521-32     Citation Subset:  IM    
Department of Chemical Engineering, University of Waterloo, 200 University Avenue West, Waterloo, ON, Canada, N2L 3G1.
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MeSH Terms
Bacteriological Techniques
Biotechnology / methods
Escherichia coli / genetics*,  metabolism*
Gene Expression
Heat-Shock Response
Protein Engineering / methods*
Protein Folding
Recombinant Proteins / biosynthesis*,  chemistry,  genetics*
Reg. No./Substance:
0/Recombinant Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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