| Engineering of affibody molecules for therapy and diagnostics. | |
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MedLine Citation:
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PMID: 22735949 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Affibody molecules are small and robust non-immunoglobulin affinity ligands capable of binding to a wide range of protein targets. They are selected from combinatorial libraries based on a 58 amino acid, three-alpha-helical Z-domain scaffold. They share no sequence or structural homologies to antibodies and in contrast to antibodies they can be functionally produced both by peptide synthesis and by recombinant expression in Escherichia coli. Protein engineering is used to adapt Affibody molecules binding to a target of interest to the specific demands imposed by the intended application. Obviously, the optimal molecule for molecular imaging will be different from the optimal molecule for therapy. Here, we describe general strategies to optimize Affibody molecules for diagnostic imaging and therapy applications. |
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Authors:
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Joachim Feldwisch; Vladimir Tolmachev |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Methods in molecular biology (Clifton, N.J.) Volume: 899 ISSN: 1940-6029 ISO Abbreviation: Methods Mol. Biol. Publication Date: 2012 |
Date Detail:
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Created Date: 2012-06-27 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9214969 Medline TA: Methods Mol Biol Country: United States |
Other Details:
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Languages: eng Pagination: 103-26 Citation Subset: IM |
Affiliation:
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Affibody AB, Solna, Sweden, joachim.feldwisch@affibody.com. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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