Document Detail

Engineering Propionibacterium acidipropionici for enhanced propionic acid tolerance and fermentation.
MedLine Citation:
PMID:  19530125     Owner:  NLM     Status:  MEDLINE    
Propionibacterium acidipropionici, a Gram-positive, anaerobic bacterium, has been the most used species for propionic acid production from sugars. In this study, the metabolically engineered mutant ACK-Tet, which has its acetate kinase gene knocked out from the chromosome, was immobilized and adapted in a fibrous bed bioreactor (FBB) to increase its acid tolerance and ability to produce propionic acid at a high final concentration in fed-batch fermentation. After about 3 months adaptation in the FBB, the propionic acid concentration in the fermentation broth reached approximately 100 g/L, which was much higher than the highest concentration of approximately 71 g/L previously attained with the wild-type in the FBB. To understand the mechanism and factors contributing to the enhanced acid tolerance, adapted mutant cells were harvested from the FBB and characterized for their morphology, growth inhibition by propionic acid, protein expression profiles as observed in SDS-PAGE, and H+-ATPase activity, which is related to the proton pumping and cell's ability to control its intracellular pH gradient. The adapted mutant obtained from the FBB showed significantly reduced growth sensitivity to propionic acid inhibition, increased H+-ATPase expression and activity, and significantly elongated rod morphology.
An Zhang; Shang-Tian Yang
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biotechnology and bioengineering     Volume:  104     ISSN:  1097-0290     ISO Abbreviation:  Biotechnol. Bioeng.     Publication Date:  2009 Nov 
Date Detail:
Created Date:  2009-09-28     Completed Date:  2009-11-24     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7502021     Medline TA:  Biotechnol Bioeng     Country:  United States    
Other Details:
Languages:  eng     Pagination:  766-73     Citation Subset:  IM    
Copyright Information:
Copyright 2009 Wiley Periodicals, Inc.
William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, 140 West 19th Avenue, Columbus, Ohio 43210, USA.
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MeSH Terms
Anti-Bacterial Agents / metabolism*,  pharmacology
Bioreactors / microbiology
Cells, Immobilized
Drug Tolerance*
Genetic Engineering*
Propionibacterium / cytology,  enzymology,  genetics*,  metabolism*
Propionic Acids / metabolism*,  pharmacology
Proton-Translocating ATPases / metabolism
Reg. No./Substance:
0/Anti-Bacterial Agents; 0/Propionic Acids; 79-09-4/propionic acid; EC ATPases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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