Document Detail

Engineering musculoskeletal tissues with human embryonic germ cell derivatives.
MedLine Citation:
PMID:  20178108     Owner:  NLM     Status:  MEDLINE    
The cells derived from differentiating embryoid bodies of human embryonic germ (hEG) cells express a broad spectrum of gene markers and have been induced toward ecto- and endodermal lineages. We describe here in vitro and in vivo differentiation of hEG-derived cells (LVEC line) toward mesenchymal tissues. The LVEC cells express many surface marker proteins characteristic of mesenchymal stem cells and differentiated into cartilage, bone, and fat. Homogenous hyaline cartilage was generated from cells after 63 population doublings. In vivo results demonstrate cell survival, differentiation, and tissue formation. The high proliferative capacity of hEG-derived cells and their ability to differentiate and form three-dimensional mesenchymal tissues without teratoma formation underscores their significant potential for regenerative medicine. The adopted coculture system also provides new insights into how a microenvironment comprised of extracellular and cellular components may be harnessed to generate hierarchically complex tissues from pluripotent cells.
Shyni Varghese; Nathaniel S Hwang; Angela Ferran; Alexander Hillel; Parnduangjai Theprungsirikul; Adam C Canver; Zijun Zhang; John Gearhart; Jennifer Elisseeff
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Stem cells (Dayton, Ohio)     Volume:  28     ISSN:  1549-4918     ISO Abbreviation:  Stem Cells     Publication Date:  2010 Apr 
Date Detail:
Created Date:  2010-04-21     Completed Date:  2010-06-18     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9304532     Medline TA:  Stem Cells     Country:  United States    
Other Details:
Languages:  eng     Pagination:  765-74     Citation Subset:  IM    
Department of Biomedical Engineering, Johns Hopkins University, Baltimore, Maryland, USA.
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MeSH Terms
Cell Differentiation
Cell Lineage
Cells, Cultured
Embryo, Mammalian / cytology*,  metabolism*
Gene Expression Regulation
Germ Cells / cytology*,  metabolism*
Muscle, Skeletal / cytology*,  metabolism*
Tissue Engineering / methods*

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