Document Detail

Engineering the microstructure of electrospun fibrous scaffolds by microtopography.
MedLine Citation:
PMID:  23534553     Owner:  NLM     Status:  MEDLINE    
Controlling the structure and organization of electrospun fibers is desirable for fabricating scaffolds and materials with defined microstructures. However, the effects of microtopography on the deposition and, in turn, the organization of the electrospun fibers are not well understood. In this study, conductive polydimethylsiloxane (PDMS) templates with different micropatterns were fabricated by combining photolithography, silicon wet etching, and PDMS molding techniques. The fiber organization was varied by fine-tuning the microtopography of the electrospinning collector. Fiber conformity and alignment were influenced by the depth and the slope of microtopography features, resulting in scaffolds comprising either an array of microdomains with different porosity and fiber alignment or an array of microwells. Microtopography affected the fiber organization for hundreds of micrometers below the scaffold surface, resulting in scaffolds with distinct surface properties on each side. In addition, the fiber diameter was also affected by the fiber conformity. The effects of the fiber arrangement in the scaffolds on the morphology, migration, and infiltration of cells were examined by in vitro and in vivo experiments. Cell morphology and organization were guided by the fibers in the microdomains, and cell migration was enhanced by the aligned fibers and the three-dimensional scaffold structure. Cell infiltration was correlated with the microdomain porosity. Microscale control of the fiber organization and the porosity at the surface and through the thickness of the fibrous scaffolds, as demonstrated by the results of this study, provides a powerful means of engineering the three-dimensional structure of electrospun fibrous scaffolds for cell and tissue engineering.
Qian Cheng; Benjamin L-P Lee; Kyriakos Komvopoulos; Song Li
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2013-04-25
Journal Detail:
Title:  Biomacromolecules     Volume:  14     ISSN:  1526-4602     ISO Abbreviation:  Biomacromolecules     Publication Date:  2013 May 
Date Detail:
Created Date:  2013-05-13     Completed Date:  2013-12-16     Revised Date:  2014-06-18    
Medline Journal Info:
Nlm Unique ID:  100892849     Medline TA:  Biomacromolecules     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1349-60     Citation Subset:  IM    
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MeSH Terms
Biocompatible Materials / chemical synthesis*,  pharmacology
Cell Line
Cell Movement / drug effects
Cell Proliferation / drug effects
Dimethylpolysiloxanes / chemistry*
Electrochemical Techniques
Lactic Acid / chemistry*
Mesenchymal Stromal Cells / cytology,  drug effects*,  physiology
Microscopy, Electron, Scanning
Polymers / chemistry*
Surface Properties
Tissue Engineering
Tissue Scaffolds*
Grant Support
Reg. No./Substance:
0/Biocompatible Materials; 0/Dimethylpolysiloxanes; 0/Polymers; 26100-51-6/poly(lactic acid); 33X04XA5AT/Lactic Acid; 63148-62-9/baysilon

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