| Engineering Escherichia coli for production of C₁₂-C₁₄ polyhydroxyalkanoate from glucose. | |
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MedLine Citation:
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PMID: 23141473 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Demand for sustainable materials motivates the development of microorganisms capable of synthesizing products from renewable substrates. A challenge to commercial production of polyhydroxyalkanoates (PHA), microbially derived polyesters, is engineering metabolic pathways to produce a polymer with the desired monomer composition from an unrelated and renewable source. Here, we demonstrate a metabolic pathway for converting glucose into medium-chain-length (mcl)-PHA composed primarily of 3-hydroxydodecanoate monomers. This pathway combines fatty acid biosynthesis, an acyl-ACP thioesterase to generate desired C₁₂ and C₁₄ fatty acids, β-oxidation for conversion of fatty acids to (R)-3-hydroxyacyl-CoAs, and a PHA polymerase. A key finding is that Escherichia coli expresses multiple copies of enzymes involved in β-oxidation under aerobic conditions. To produce polyhydroxydodecanoate, an acyl-ACP thioesterase (BTE), an enoyl-CoA hydratase (phaJ3), and mcl-PHA polymerase (phaC2) were overexpressed in E. coli ΔfadRABIJ. Yields were improved through expression of an acyl-CoA synthetase resulting in production over 15% CDW--the highest reported production of mcl-PHA of a defined composition from an unrelated carbon source. |
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Authors:
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Daniel E Agnew; Amanda K Stevermer; J Tyler Youngquist; Brian F Pfleger |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Metabolic engineering Volume: 14 ISSN: 1096-7184 ISO Abbreviation: Metab. Eng. Publication Date: 2012 Nov |
Date Detail:
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Created Date: 2012-11-12 Completed Date: 2013-05-09 Revised Date: 2013-05-15 |
Medline Journal Info:
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Nlm Unique ID: 9815657 Medline TA: Metab Eng Country: Belgium |
Other Details:
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Languages: eng Pagination: 705-13 Citation Subset: IM |
Copyright Information:
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Copyright © 2012 Elsevier Inc. All rights reserved. |
Affiliation:
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Department of Chemical and Biological Engineering, University of Wisconsin-Madison, Madison, WI 53706, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Cloning, Molecular
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methods Escherichia coli / metabolism* Glucose / metabolism* Multienzyme Complexes / genetics, metabolism* Polyhydroxyalkanoates / genetics, isolation & purification, metabolism* Protein Engineering / methods* Pseudomonas aeruginosa / physiology* Recombinant Proteins / metabolism |
| Grant Support | |
ID/Acronym/Agency:
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5T32GM08349/GM/NIGMS NIH HHS; T32 GM008349/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Multienzyme Complexes; 0/Polyhydroxyalkanoates; 0/Recombinant Proteins; 50-99-7/Glucose |
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