| Engineering Designer Transcription Activator--Like Effector Nucleases (TALENs) by REAL or REAL-Fast Assembly. | |
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MedLine Citation:
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PMID: 23026907 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Engineered transcription activator-like effector nucleases (TALENs) are broadly useful tools for performing targeted genome editing in a wide variety of organisms and cell types including plants, zebrafish, C. elegans, rat, human somatic cells, and human pluripotent stem cells. Here we describe detailed protocols for the serial, hierarchical assembly of TALENs that require neither PCR nor specialized multi-fragment ligations and that can be implemented by any laboratory. These restriction enzyme and ligation (REAL)-based protocols can be practiced using plasmid libraries and user-friendly, Web-based software that both identifies target sites in sequences of interest and generates printable graphical guides that facilitate assembly of TALENs. With the described platform of reagents, protocols, and software, researchers can easily engineer multiple TALENs within 2 weeks using standard cloning techniques. Curr. Protoc. Mol. Biol. 100:12.15.1-12.15.14. © 2012 by John Wiley & Sons, Inc. |
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Authors:
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Deepak Reyon; Cyd Khayter; Maureen R Regan; J Keith Joung; Jeffry D Sander |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Current protocols in molecular biology / edited by Frederick M. Ausubel ... [et al.] Volume: Chapter 12 ISSN: 1934-3647 ISO Abbreviation: Curr Protoc Mol Biol Publication Date: 2012 Oct |
Date Detail:
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Created Date: 2012-10-02 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8908160 Medline TA: Curr Protoc Mol Biol Country: United States |
Other Details:
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Languages: eng Pagination: Unit12.15 Citation Subset: IM |
Affiliation:
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Molecular Pathology Unit, Center for Computational and Integrative Biology and Center for Cancer Research, Massachusetts General Hospital, Charlestown, Massachusetts. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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