Document Detail


Engineering Cyclic Amidases for Non-natural Amino Acid Synthesis.
MedLine Citation:
PMID:  21956558     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Hydantoinases/dihydropyrimidinases are important biotechnological enzymes involved in the production of α- and β-amino acids. Their isolation from new sources with different substrate specificities, improved activity, enantioselectivity, or higher stability continues to be of great industrial interest. Here, we provide a detailed description of how to produce high quantities of the recombinant hydantoinase/dihydropyrimidinase enzyme from Sinorhizobium meliloti CECT4114 (SmeDhp). Several techniques are combined to obtain this goal, from cloning to activity measurement by HPLC.
Authors:
Francisco Javier Las Heras-Vázquez; Josefa María Clemente-Jiménez; Sergio Martínez-Rodríguez; Felipe Rodríguez-Vico
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  794     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2012  
Date Detail:
Created Date:  2011-09-29     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  87-104     Citation Subset:  IM    
Affiliation:
Departamento de Química-Física, Bioquímica y Química Inorgánica, Universidad de Almería, Almería, Spain, fjheras@ual.es.
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