Document Detail


Engineered metal binding sites on green fluorescence protein.
MedLine Citation:
PMID:  10679227     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The ability to assay a variety of metals by noninvasive methods has applications in both biomedical and environmental research. Green fluorescent protein (GFP) is a protein isolated from coelenterates that exhibits spontaneous fluorescence. GFP does not require any exogenous cofactors for fluorescence, and can be easily appended to other proteins at the DNA level, producing a fluorescence-labeled target protein in vivo. Metals in close proximity to chromophores are known to quench fluorescence in a distance-dependent fashion. Potential metal binding sites on the surface of GFP have been identified and mutant proteins have been designed, created, and characterized. These metal-binding mutants of GFP exhibit fluorescence quenching at lower transition metal ion concentrations than those of the wild-type protein. These GFP mutants represent a new class of protein-based metal sensors.
Authors:
T A Richmond; T T Takahashi; R Shimkhada; J Bernsdorf
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  268     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2000 Feb 
Date Detail:
Created Date:  2000-03-10     Completed Date:  2000-03-10     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  462-5     Citation Subset:  IM    
Copyright Information:
Copyright 2000 Academic Press.
Affiliation:
Joint Science Department, Claremont McKenna, Pitzer, and Scripps Colleges, 925 North Mills Avenue, Claremont, California 91711, USA. richmond@jsd.claremont.edu
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MeSH Terms
Descriptor/Qualifier:
Binding Sites
Green Fluorescent Proteins
Luminescent Proteins / genetics,  metabolism*
Metals / metabolism*
Models, Molecular
Mutagenesis, Site-Directed
Protein Engineering / methods
Chemical
Reg. No./Substance:
0/Luminescent Proteins; 0/Metals; 147336-22-9/Green Fluorescent Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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