Document Detail


Engineered cell culture substrates for axon guidance studies: moving beyond proof of concept.
MedLine Citation:
PMID:  23288417     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Promoting axon regeneration following injury is one of the ultimate challenges of neuroscience, and understanding the mechanisms that regulate axon growth and guidance is essential to achieve this goal. During development axons are directed over relatively long distances by a precise extracellular distribution of chemical signals in the embryonic nervous system. Multiple guidance proteins, including netrins, slits, semaphorins, ephrins and neurotrophins have been identified as key players in this process. During the last decade, engineered cell culture substrates have been developed to investigate the cellular and molecular mechanisms underlying axon guidance. This review is focused on the biological insights that have been achieved using new techniques that attempt to mimic in vitro the spatial patterns of proteins that growth cones encounter in vivo.
Authors:
Joannie Roy; Timothy E Kennedy; Santiago Costantino
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Review    
Journal Detail:
Title:  Lab on a chip     Volume:  13     ISSN:  1473-0189     ISO Abbreviation:  Lab Chip     Publication Date:  2013 Feb 
Date Detail:
Created Date:  2013-01-24     Completed Date:  2013-10-30     Revised Date:  2013-12-24    
Medline Journal Info:
Nlm Unique ID:  101128948     Medline TA:  Lab Chip     Country:  England    
Other Details:
Languages:  eng     Pagination:  498-508     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Axons / metabolism*
Cell Culture Techniques / methods*
Humans
Nerve Tissue Proteins / chemistry,  metabolism
Neural Stem Cells / cytology,  metabolism
Neurosciences
Tissue Engineering
Chemical
Reg. No./Substance:
0/Nerve Tissue Proteins
Comments/Corrections
Erratum In:
Lab Chip. 2013 Dec 21;13(24):4890

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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