Document Detail

Engineered Constitutive Pathway in Klebsiella pneumoniae for 3-Hydroxypropionic Acid Production and Implications for Decoupling Glycerol Dissimilation Pathways.
MedLine Citation:
PMID:  23192305     Owner:  NLM     Status:  Publisher    
3-Hydroxypropionic acid (3-HP) is a commercially important platform chemical from which a panel of chemicals can be generated. Klebsiella pneumoniae has been regarded as a promising host strain in glycerol-based 3-HP production for its exceptional ability to metabolize glycerol. Since the glycerol dissimilation mechanism governs the carbon flux distribution from glycerol, inducible strong promoters were usually employed to enhance the glycerol consumption and 3-HP production. Here, we report an alternative strategy that the native promoter of dhaB gene was applied to enhance 3-HP production in K. pneumoniae. The key enzyme genes (ald4 and dhaB) for 3-HP biosynthesis were co-expressed under this promoter. Metabolic analysis revealed that the 3-HP formation was partially coupled with cell metabolism. To optimize the production of 3-HP, the effects of glucose as energy source assistant were investigated based on the analysis of fermentation process kinetics. The highest 3-HP yield (3.77 g/L in flask) was observed upon optimized conditions. Since there were no additional inducers needed, the strategy of employing native promoter seems more feasible to industrial application. More importantly, the employment of constitutive promoter demonstrated an effective approach for decoupling the natural correlation between respiratory metabolism and glycerol dissimilation in K. pneumoniae.
Xi Wang; Na Sa; Feng-Huan Wang; Ping-Fang Tian
Related Documents :
23192305 - Engineered constitutive pathway in klebsiella pneumoniae for 3-hydroxypropionic acid pr...
24301055 - Production of apolipoprotein c-iii knockout rabbits using zinc finger nucleases.
24298215 - A study on the effect of metabolic heat generation on biological tissue freezing.
22445855 - Direct effects of arsenic trioxide on action potentials in isolated cardiac tissues: im...
10518325 - Dietary change and stable isotopes: a model of growth and dormancy in cave bears.
22342725 - Metabolism of chlorobiphenyls by a variant biphenyl dioxygenase exhibiting enhanced act...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-11-29
Journal Detail:
Title:  Current microbiology     Volume:  -     ISSN:  1432-0991     ISO Abbreviation:  Curr. Microbiol.     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-11-29     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7808448     Medline TA:  Curr Microbiol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Beijing Key Lab of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, 100029, People's Republic of China.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Adherence of Brachyspira hyodysenteriae to Porcine Intestinal Epithelial Cells is Inhibited by Antib...
Next Document:  6,6'-Dihydroxy terpyridine: a proton-responsive bifunctional ligand and its application in catalytic...