Document Detail


Engagement of CD31 delivers an activating signal that contributes to the survival of chronic lymphocytic leukaemia cells.
MedLine Citation:
PMID:  20813004     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The present study showed that engagement of CD31 delivers a survival signal in chronic lymphocytic leukaemia (CLL) cells. We describe two groups of CLL, showing different kinetics of apoptosis in vitro and distinct ratios between anti-apoptotic and pro-apoptotic proteins: CLL-I displayed low Bcl-x(L) /Bax and Bcl-2/Bax ratio and underwent rapid apoptosis in vitro; CLL-II had high Bcl-x(L) /Bax and Bcl-2/Bax ratio and were resistant to apoptosis for several days. Nurse-like cells, expressing vimentin, CD68 and CD31 were detected mainly in CLL-II cultures. Of note, CD31 cross-linking, obtained with a specific monoclonal antibody (mAb), induced phosphatidylinositol-3-kinase-dependent Akt phosphorylation and nuclear translocation of the nuclear factor-kBp65 and p52 subunits in both CLL groups, leading to upregulation of Bcl-2 and Bcl-x(L) transcription and increased cell survival. Binding to CD31(+) stable transfectants, could also deliver an anti-apoptotic signal in B cells of both CLL-I and CLL-II, increasing the Bcl-2 and Bcl-x(L) protein content, regardless the expression of CD38. On the other hand, the addition of the F(ab')₂ (that is unable to oligomerize the target molecule) of the anti-CD31 mAb prevented these effects. These data suggest that the CD31 adhesion system may play a role also in vivo in maintaining CLL survival.
Authors:
Alessandro Poggi; Claudia Prevosto; Silvia Catellani; Ilaria Rocco; Anna Garuti; Maria R Zocchi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-08-31
Journal Detail:
Title:  British journal of haematology     Volume:  151     ISSN:  1365-2141     ISO Abbreviation:  Br. J. Haematol.     Publication Date:  2010 Nov 
Date Detail:
Created Date:  2010-10-15     Completed Date:  2010-12-06     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372544     Medline TA:  Br J Haematol     Country:  England    
Other Details:
Languages:  eng     Pagination:  252-64     Citation Subset:  IM    
Copyright Information:
© 2010 Blackwell Publishing Ltd.
Affiliation:
Laboratory of Molecular Oncology and Angiogenesis, National Institute for Cancer Research, University of Genoa, Italy. alessandro.poggi@istge.it
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MeSH Terms
Descriptor/Qualifier:
Aged
Aged, 80 and over
Antibodies, Heterophile / physiology
Antigens, CD31 / physiology*
Antigens, CD38 / metabolism
Apoptosis / physiology
Cell Adhesion / physiology
Cell Survival / physiology
Female
Humans
Leukemia, Lymphocytic, Chronic, B-Cell / metabolism*,  pathology
Male
Membrane Glycoproteins / metabolism
Middle Aged
Neoplasm Proteins / metabolism
Signal Transduction / physiology
Tumor Cells, Cultured
Chemical
Reg. No./Substance:
0/Antibodies, Heterophile; 0/Antigens, CD31; 0/Membrane Glycoproteins; 0/Neoplasm Proteins; EC 3.2.2.5/Antigens, CD38; EC 3.2.2.5/CD38 protein, human

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