| Engagement of CD31 delivers an activating signal that contributes to the survival of chronic lymphocytic leukaemia cells. | |
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MedLine Citation:
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PMID: 20813004 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The present study showed that engagement of CD31 delivers a survival signal in chronic lymphocytic leukaemia (CLL) cells. We describe two groups of CLL, showing different kinetics of apoptosis in vitro and distinct ratios between anti-apoptotic and pro-apoptotic proteins: CLL-I displayed low Bcl-x(L) /Bax and Bcl-2/Bax ratio and underwent rapid apoptosis in vitro; CLL-II had high Bcl-x(L) /Bax and Bcl-2/Bax ratio and were resistant to apoptosis for several days. Nurse-like cells, expressing vimentin, CD68 and CD31 were detected mainly in CLL-II cultures. Of note, CD31 cross-linking, obtained with a specific monoclonal antibody (mAb), induced phosphatidylinositol-3-kinase-dependent Akt phosphorylation and nuclear translocation of the nuclear factor-kBp65 and p52 subunits in both CLL groups, leading to upregulation of Bcl-2 and Bcl-x(L) transcription and increased cell survival. Binding to CD31(+) stable transfectants, could also deliver an anti-apoptotic signal in B cells of both CLL-I and CLL-II, increasing the Bcl-2 and Bcl-x(L) protein content, regardless the expression of CD38. On the other hand, the addition of the F(ab')₂ (that is unable to oligomerize the target molecule) of the anti-CD31 mAb prevented these effects. These data suggest that the CD31 adhesion system may play a role also in vivo in maintaining CLL survival. |
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Authors:
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Alessandro Poggi; Claudia Prevosto; Silvia Catellani; Ilaria Rocco; Anna Garuti; Maria R Zocchi |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-08-31 |
Journal Detail:
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Title: British journal of haematology Volume: 151 ISSN: 1365-2141 ISO Abbreviation: Br. J. Haematol. Publication Date: 2010 Nov |
Date Detail:
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Created Date: 2010-10-15 Completed Date: 2010-12-06 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0372544 Medline TA: Br J Haematol Country: England |
Other Details:
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Languages: eng Pagination: 252-64 Citation Subset: IM |
Copyright Information:
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© 2010 Blackwell Publishing Ltd. |
Affiliation:
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Laboratory of Molecular Oncology and Angiogenesis, National Institute for Cancer Research, University of Genoa, Italy. alessandro.poggi@istge.it |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Aged Aged, 80 and over Antibodies, Heterophile / physiology Antigens, CD31 / physiology* Antigens, CD38 / metabolism Apoptosis / physiology Cell Adhesion / physiology Cell Survival / physiology Female Humans Leukemia, Lymphocytic, Chronic, B-Cell / metabolism*, pathology Male Membrane Glycoproteins / metabolism Middle Aged Neoplasm Proteins / metabolism Signal Transduction / physiology Tumor Cells, Cultured |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Heterophile; 0/Antigens, CD31; 0/Membrane Glycoproteins; 0/Neoplasm Proteins; EC 3.2.2.5/Antigens, CD38; EC 3.2.2.5/CD38 protein, human |
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