| Energy transfer efficiency on lymphocytes of leukemia patients measured by fluorescence polarization. | |
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MedLine Citation:
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PMID: 15621788 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Measurements of fluorescence resonance energy transfer efficiency (E), via fluorescence polarization, have been applied to distinguish between chronic lymphocytic leukemia (CLL) patients and healthy persons. Capping of Concanavalin-A receptors is more pronounced in normal lymphocytes than in those of CLL patients. Membrane capping decreases the distance between donor and acceptor molecules embedded in the membrane (r), and thus increases the monitored energy transfer efficiency (E approximately 1/r6). Blood samples of 10 healthy subjects and 16 CLL patients were examined. In the healthy subjects, the mean E value for capped lymphocytes was 19 +/- 3, whereas in the CLL patients it was significantly lower (8 +/- 5) (P < 0.01). |
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Authors:
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Meir Cohen-Kashi; Michal Haran; Elena Afrimzon; Mordechai Deutsch |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Leukemia & lymphoma Volume: 46 ISSN: 1042-8194 ISO Abbreviation: Leuk. Lymphoma Publication Date: 2005 Jan |
Date Detail:
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Created Date: 2004-12-28 Completed Date: 2005-06-01 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9007422 Medline TA: Leuk Lymphoma Country: England |
Other Details:
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Languages: eng Pagination: 113-20 Citation Subset: IM |
Affiliation:
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Biophysical Interdisciplinary Schottenstein Center for the Research and the Technology of the Cellome, Physics Department, Bar-Ilan University, Ramat-Gan, Israel. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Concanavalin A
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analysis,
metabolism* Female Fluorescence Polarization Fluorescence Resonance Energy Transfer* Humans Leukemia / blood, diagnosis*, metabolism* Lymphocytes / metabolism*, pathology Male Phenotype Random Allocation |
| Chemical | |
Reg. No./Substance:
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11028-71-0/Concanavalin A |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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