| Energy thresholds that determine membrane integrity and injury in a renal epithelial cell line (LLC-PK1). Relationships to phospholipid degradation and unesterified fatty acid accumulation. | |
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MedLine Citation:
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PMID: 3125228 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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This study related ATP levels with membrane damage, lipid abnormalities, and cell death in energy-depleted LLC-PK1 cells. Oxidative phosphorylation was inhibited by antimycin A, and glycolysis was regulated by graded glucose deprivation to achieve stepwise ATP depletion. Over a range of ATP levels down to approximately equal to 5% of normal, over 5 h, cells were altered only minimally, or injured reversibly. Such cells maintained mitochondrial potential, and retained more K+ than cells without an energy source. Over the same duration, cells without an energy source were lethally injured. Treatment with antimycin induced increments of triglycerides and decreases of phospholipids. With severe ATP depletion (approximately equal to 5-10% of normal after 5 h), decrease of phospholipids was marked. Cells in which ATP was not measurable (or was less than 5% of normal) showed comparable phospholipid declines but, in addition, showed massive and progressive increase of unesterified fatty acids. The results identified a low threshold of ATP, at best 5-10% of normal, which preserved viability in LLC-PK1 cells despite major loss of membrane phospholipids. This threshold also determined the ability of cells to maintain their normally low levels of unesterified fatty acids. Failure of energy-dependent mechanisms that normally metabolize unesterified fatty acids may be a correlate of the extent of energy depletion that determines lethal injury. |
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Authors:
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M A Venkatachalam; Y J Patel; J I Kreisberg; J M Weinberg |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: The Journal of clinical investigation Volume: 81 ISSN: 0021-9738 ISO Abbreviation: J. Clin. Invest. Publication Date: 1988 Mar |
Date Detail:
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Created Date: 1988-04-07 Completed Date: 1988-04-07 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 7802877 Medline TA: J Clin Invest Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 745-58 Citation Subset: AIM; IM |
Affiliation:
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Department of Pathology, University of Texas Health Science Center, San Antonio 78284. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acetates
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metabolism Adenosine Triphosphate / metabolism Animals Arachidonic Acid Arachidonic Acids / metabolism Cell Line Cell Membrane / metabolism*, pathology, ultrastructure Cell Survival* Culture Media Energy Metabolism* Epithelium Fatty Acids, Nonesterified / metabolism* Fluorescent Dyes Kidney Lactates / metabolism Lactic Acid Mitochondria / metabolism Phospholipids / metabolism* Potassium / metabolism Rhodamine 123 Rhodamines / pharmacology Swine |
| Grant Support | |
ID/Acronym/Agency:
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AM-29787/AM/NIADDK NIH HHS; AM-37139/AM/NIADDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Acetates; 0/Arachidonic Acids; 0/Culture Media; 0/Fatty Acids, Nonesterified; 0/Fluorescent Dyes; 0/Lactates; 0/Phospholipids; 0/Rhodamines; 50-21-5/Lactic Acid; 506-32-1/Arachidonic Acid; 56-65-5/Adenosine Triphosphate; 62669-70-9/Rhodamine 123; 7440-09-7/Potassium |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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