Document Detail

Energy substrate requirements for in-vitro development of hamster 1- and 2-cell embryos to the blastocyst stage.
MedLine Citation:
PMID:  1874959     Owner:  NLM     Status:  MEDLINE    
Energy substrate requirements (pyruvate, lactate and amino acids) were determined for in-vitro development of hamster 1- and 2-cell embryos to blastocysts, using a chemically defined, protein free medium (hamster embryo culture medium, HECM). One-cell embryos were very sensitive to energy substrate type and concentration. Pyruvate alone could not support development of 1-cell embryos to greater than 4-cells, whereas lactate as sole energy substrate supported 14% development into morulae/blastocysts. Pyruvate, with lactate and 20 amino acids, inhibited 1-cell embryo development into blastocysts relative to lactate and 20 amino acids. The highest development of 1-cell embryos to blastocysts (up to 27%) occurred with reduced lactate concentration (less than 10 mM) and either 20 amino acids or 0.2 mM glutamine. Hamster 2-cell embryos were much less sensitive to energy substrates, requiring only lactate for development to blastocysts (53%). Lactate with 20 amino acids supported 70-75% of 2-cell embryos to blastocysts. Glutamine as sole energy and nitrogen source supported development to morulae and blastocysts of some 2-cell, but not 1-cell, embryos. Pyruvate did not enhance development of 2-cell embryos. We conclude that (i) altering the types and concentrations of available energy substrates drastically changes the developmental responses of 1-cell hamster embryos in vitro and (ii) energy substrate requirements for hamster embryo development in vitro are markedly different from those of mouse embryos, the standard model for studies on preimplantation development. This is the first report of successful in-vitro culture of hamster 1-cell embryos to the blastocyst stage.
S H McKiernan; B D Bavister; R J Tasca
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Human reproduction (Oxford, England)     Volume:  6     ISSN:  0268-1161     ISO Abbreviation:  Hum. Reprod.     Publication Date:  1991 Jan 
Date Detail:
Created Date:  1991-09-26     Completed Date:  1991-09-26     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8701199     Medline TA:  Hum Reprod     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  64-75     Citation Subset:  IM    
Department of Veterinary Science, University of Wisconsin, Madison 53706.
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MeSH Terms
Amino Acids / metabolism,  pharmacology
Blastocyst / metabolism*
Cleavage Stage, Ovum / drug effects*,  metabolism
Culture Media / chemistry*
Culture Techniques / methods
Dose-Response Relationship, Drug
Energy Metabolism*
Glutamine / metabolism,  pharmacology
Lactates / metabolism,  pharmacology
Lactic Acid
Morula / drug effects,  metabolism
Pyruvates / metabolism,  pharmacology
Pyruvic Acid
Grant Support
Reg. No./Substance:
0/Amino Acids; 0/Culture Media; 0/Lactates; 0/Pyruvates; 127-17-3/Pyruvic Acid; 50-21-5/Lactic Acid; 56-85-9/Glutamine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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