| Energy restriction and exercise modulate angiopoietins and vascular endothelial growth factor expression in the cavernous tissue of high-fat diet-fed rats. | |
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MedLine Citation:
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PMID: 22138901 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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The purpose of the current study was to evaluate the effect of a high-fat (HF) diet, energy restriction and exercise on the expression of vascular endothelial growth factor (VEGF), angiopoietin (Ang) 1 and 2, and their receptors in rat corpus cavernosum (CC). Male Wistar rats were fed ad libitum with an HF diet for 8 or 16 weeks. After 8 weeks of the HF diet, a group of rats was subjected to energy restriction with or without exercise for 8 weeks. Control animals had free access to standard diet for the same period. After euthanasia, blood was collected and the penises removed for immunofluorescence assays (VEGF, VEGF receptor (VEGFR) 1 and 2, Ang1, Ang2 and Tie2) and semiquantification of VEGF, VEGFR1, VEGFR2, Ang1, Ang2, Tie2, endothelial nitric oxide synthase (eNOS) and Akt/phospho-Akt by Western blotting. HF diet-fed rats exhibited lower high-density lipoprotein cholesterol (HDL-c) levels, higher systolic blood pressure and an increased atherogenic index. A significant increase in Ang2 expression in the CC was verified and coupled to a decrease in VEGF and VEGFRs. The Akt pathway was activated by the HF diet. Energy restriction and exercise increased eNOS expression and restored most HF diet-induced modifications except for VEGFR2 expression. These results emphasize the role of diet on vascular function regulation, demonstrating that cavernous imbalance of VEGF/VEGFRs and Angs/Tie2 systems occurs before serum lipid changes and obesity onset, antedating structural atherosclerotic features.Asian Journal of Andrology advance online publication, 5 December 2011; doi:10.1038/aja.2011.131. |
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Authors:
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Inês Tomada; Nuno Tomada; Henrique Almeida; Delminda Neves |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-12-05 |
Journal Detail:
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Title: Asian journal of andrology Volume: - ISSN: 1745-7262 ISO Abbreviation: - Publication Date: 2011 Dec |
Date Detail:
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Created Date: 2011-12-5 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100942132 Medline TA: Asian J Androl Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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1] Department of Experimental Biology, Faculty of Medicine, Universidade do Porto-Alameda Prof, Hernâni Monteiro, Porto 4200-319, Portugal [2] Instituto de Biologia Molecular e Celular (IBMC), Universidade do Porto-Rua do Campo Alegre 823, Porto 4150-180, Portugal. |
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