| Energy metabolism of the trophectoderm and inner cell mass of the mouse blastocyst. | |
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MedLine Citation:
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PMID: 8228868 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The two cell populations of the day 5 mouse blastocyst--the inner cell mass (ICM) and trophectoderm (TE)--were isolated by immunosurgery and TE biopsy. The uptake of glucose and pyruvate and the production of lactate were measured in the isolated cell populations and in single day 5 blastocysts, using a non-invasive ultramicrofluorescence technique. The number of cells in isolated ICMs, TE vesicles, and intact blastocysts were counted after differential labeling with fluorochromes. The uptake of glucose and the formation of lactate were approximately three and five times greater, respectively, in the ICM cells compared with the TE cells. All the glucose consumed by the ICM could be accounted for by lactate formation, whereas the value for TE was 55%. The uptake of pyruvate was low in both cell types. The results are consistent with the proposition that the TE acts as a transporting epithelium, sparing nutrients for metabolism by the ICM. |
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Authors:
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L C Hewitson; H J Leese |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The Journal of experimental zoology Volume: 267 ISSN: 0022-104X ISO Abbreviation: J. Exp. Zool. Publication Date: 1993 Nov |
Date Detail:
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Created Date: 1993-12-21 Completed Date: 1993-12-21 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0375365 Medline TA: J Exp Zool Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 337-43 Citation Subset: IM |
Affiliation:
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Department of Biology, University of York, Heslington, England. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Blastocyst / cytology, metabolism* Culture Techniques Ectoderm / metabolism Energy Metabolism* Female Glucose / metabolism Lactates / metabolism Lactic Acid Male Mice Mice, Inbred C57BL Mice, Inbred CBA Microscopy, Fluorescence Pyruvates / metabolism Pyruvic Acid Trophoblasts / cytology, metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Lactates; 0/Pyruvates; 127-17-3/Pyruvic Acid; 50-21-5/Lactic Acid; 50-99-7/Glucose |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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