Document Detail


Energy balance in DNFB-treated and untreated frog muscle.
MedLine Citation:
PMID:  1079537     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
1. Heat production and chemical changes were measured in untreated and dinitrofluorobenzene (DNFB)-treated muscles during isometric tetani. Levels of total creatine (Ct), free creatine, ATP, ADP, AMP, inorganic phosphate, glucose-1-phosphate, glucose-6-phosphate, fructose-6-phosphate, fructose-1,6-diphosphate, pyruvate, phosphoenolpyruvate, and lactate were measured. Changes in inosinic acid (IMP) were also measured. 2. DNFB effectively inhibited the creatine kinase reaction (Lohmann reaction). 3. Our major finding is that even after effective treatment with DNFB the observed heat plus work after 2 sec and 5 sec of stimulation is significantly greater than the enthalpy change produced by the measured chemical changes. This confirms that an unidentified exothermic process occurs during muscle contraction; this conclusion was reached previously from studies of untreated muscle. 4. The unexplained heat plus work is unlikely to be derived from glycolytic reactions since under anaerobic conditions no formation of lactate, pyruvate, phosphoenolpyruvate or fructose-1,6-diphosphate could be detected in either untreated or DNFB-treated muscles even 34 sec after a series of three 5 sec isometric tetani. 5. In the first 2 sec of stimulation the unexplained heat plus work is less in DNFB-treated muscles than in untreated muscles. However from 2 to 5 sec of stimulation the unexplained heat plus work is the same in treated and untreated muscles.
Authors:
N A Curtin; R C Woledge
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Publication Detail:
Type:  In Vitro; Journal Article    
Journal Detail:
Title:  The Journal of physiology     Volume:  246     ISSN:  0022-3751     ISO Abbreviation:  J. Physiol. (Lond.)     Publication Date:  1975 Apr 
Date Detail:
Created Date:  1975-09-05     Completed Date:  1975-09-05     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0266262     Medline TA:  J Physiol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  737-52     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Adenosine Diphosphate / metabolism
Adenosine Monophosphate / metabolism
Adenosine Triphosphate / metabolism
Anaerobiosis
Animals
Creatine Kinase / antagonists & inhibitors
Dinitrofluorobenzene / pharmacology*
Electric Stimulation
Energy Metabolism / drug effects*
Glycolysis
Hexosephosphates / metabolism
Hot Temperature
Inosine Monophosphate / metabolism
Muscle Contraction
Muscles / drug effects,  metabolism*
Nitrobenzenes / pharmacology*
Rana temporaria
Thermodynamics
Chemical
Reg. No./Substance:
0/Hexosephosphates; 0/Nitrobenzenes; 131-99-7/Inosine Monophosphate; 56-65-5/Adenosine Triphosphate; 58-64-0/Adenosine Diphosphate; 61-19-8/Adenosine Monophosphate; 70-34-8/Dinitrofluorobenzene; EC 2.7.3.2/Creatine Kinase
Comments/Corrections

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