| Energy intake and adiponectin gene expression. | |
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MedLine Citation:
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PMID: 21325106 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Hypoadiponectinemia and decreased adiponectin gene expression in white adipose tissue (WAT) have been well observed in obese subjects and animal models. However, the mechanism for obesity-associated hypoadiponectinemia is still largely unknown. To investigate the regulatory role of energy intake, dietary fat, and adiposity in adiponectin gene expression and blood adiponectin level, a series of feeding regimens was employed to manipulate energy intake and dietary fat in obese-prone C57BL/6, genetically obese ob/ob, obese-resistant A/J and peroxisome proliferator-activated receptor-α gene knockout (PPARα KO) mice. Adiponectin gene expression in WAT and circulating adiponectin levels were studied in these dietary intervention-treated mice. Our study showed that calorie restriction (CR) robustly increased adiponectin gene expression in epididymal fat and blood adiponectin levels in both low-fat (LF) and high-fat (HF) diet-fed C57BL/6 mice. Although HF pair-fed C57BL/6 mice received the same amount of calories as LF ad libitum-fed mice, HF diet clearly increased adiposity but showed no significant effects on adiponectin gene expression and blood adiponectin level. CR also significantly increased blood adiponectin levels in ob/ob and A/J mice. Neither CR nor HF feeding displayed any significant effect on blood adiponectin half-life in C57BL/6 mice. Interestingly, CR increased PPARα expression in epididymal fat of C57BL/6 mice. Low levels of blood adiponectin and adiponectin gene expression in WAT were observed in PPARα KO mice. PPARα agonist treatment increased adiponectin mRNA levels in 3T3-L1 adipocytes. Furthermore, CR failed to increase adiponectin gene expression and blood adiponectin levels in PPARα KO mice. Therefore, our study demonstrated that energy intake, not dietary fat, plays an important role in regulating adiponectin gene expression and blood adiponectin level. PPARα mediates CR-enhanced adiponectin gene expression in WAT. |
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Authors:
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Liping Qiao; Bonggi Lee; Brice Kinney; Hyung Sun Yoo; Jianhua Shao |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2011-02-15 |
Journal Detail:
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Title: American journal of physiology. Endocrinology and metabolism Volume: 300 ISSN: 1522-1555 ISO Abbreviation: Am. J. Physiol. Endocrinol. Metab. Publication Date: 2011 May |
Date Detail:
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Created Date: 2011-04-27 Completed Date: 2011-06-28 Revised Date: 2012-05-01 |
Medline Journal Info:
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Nlm Unique ID: 100901226 Medline TA: Am J Physiol Endocrinol Metab Country: United States |
Other Details:
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Languages: eng Pagination: E809-16 Citation Subset: IM |
Affiliation:
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Dept. of Pediatrics, Univ. of California San Diego, 9500 Gilman Dr., MC 0983, La Jolla, CA 92093, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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3T3 Cells Adiponectin / biosynthesis*, blood, genetics Adipose Tissue, White / metabolism, physiology Adiposity / physiology Animals Blotting, Western Caloric Restriction DNA Primers Dietary Fats / pharmacology Energy Intake / physiology* Epididymis / drug effects, metabolism Gene Expression / drug effects, physiology Half-Life Male Mice Mice, Inbred C57BL PPAR alpha / physiology PPAR gamma / metabolism Sirtuin 1 / metabolism |
| Grant Support | |
ID/Acronym/Agency:
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DK-077643/DK/NIDDK NIH HHS; DK-080418/DK/NIDDK NIH HHS; R01 DK080418-06/DK/NIDDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Adiponectin; 0/DNA Primers; 0/Dietary Fats; 0/PPAR alpha; 0/PPAR gamma; EC 3.5.1.-/Sirt1 protein, mouse; EC 3.5.1.-/Sirtuin 1 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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