Document Detail

Energetics of biological nitrogen fixation: determination of the ratio of formation of H2 to NH4+ catalysed by nitrogenase of Klebsiella pneumoniae in vivo.
MedLine Citation:
PMID:  22579     Owner:  NLM     Status:  MEDLINE    
Nitrogen fixation (Nif)-derepressed mutants of Klebsiella pneumoniae consumed, under optimum conditions, 7.5 to 8.5 mol glucose per mol N2 fixed. The nitrogenase system of these mutants catalysed the production of about 1.3 mol H2 per mol N2 reduced. Almost one-third of the energy as ATP and reductant used by nitrogenase in vivo may be lost in H2 production, since an ATP/2e ratio of approximately 4 was obtained. Nitrogenase-catalysed H2 production was not substantially suppressed by increasing the partial pressure of N2 from 0.2 atm (20 kPa) to 1 atm (101 kPa). In the absence of N2, H2 production catalysed by nitrogenase increased about threefold. It is concluded that nitrogenase-catalysed H2 production is of major importance in the overall efficiency of biological N2 fixation in vivo.
K Andersen; K T Shanmugam
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of general microbiology     Volume:  103     ISSN:  0022-1287     ISO Abbreviation:  J. Gen. Microbiol.     Publication Date:  1977 Nov 
Date Detail:
Created Date:  1978-02-23     Completed Date:  1978-02-23     Revised Date:  2009-10-27    
Medline Journal Info:
Nlm Unique ID:  0375371     Medline TA:  J Gen Microbiol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  107-22     Citation Subset:  IM    
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MeSH Terms
Hydrogen / metabolism
Hydrogen-Ion Concentration
Klebsiella pneumoniae / enzymology*
Nitrogen Fixation*
Nitrogenase / metabolism*
Quaternary Ammonium Compounds / metabolism
Reg. No./Substance:
0/Quaternary Ammonium Compounds; 1333-74-0/Hydrogen; EC

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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