| Energetic aspects of locked nucleic acids quadruplex association and dissociation. | |
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MedLine Citation:
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PMID: 16944520 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The design of modified nucleic acid aptamers is improved by considering thermodynamics and kinetics of their association/dissociation processes. Locked Nucleic Acids (LNA) is a promising class of nucleic acid analogs. In this work the thermodynamic and kinetic properties of a LNA quadruplex formed by the TGGGT sequence, containing only conformationally restricted LNA residues, are reported and compared to those of 2'-OMe-RNA (O-RNA) and DNA quadruplexes. The thermodynamic analysis indicates that the sugar-modified quadruplexes (LNA and O-RNA) are stabilized by entropic effects. The kinetic analysis shows that LNA and O-RNA quadruplexes are characterized by a slower dissociation and a faster association with respect to DNA quadruplex. Interestingly, the LNA quadruplex formation process shows a second-order kinetics with respect to single strand concentration and has a negative activation energy. To explain these data, a mechanism for tetramer formation with two intermediate states was proposed. |
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Authors:
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Luigi Petraccone; Eva Erra; Antonio Randazzo; Concetta Giancola |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biopolymers Volume: 83 ISSN: 0006-3525 ISO Abbreviation: Biopolymers Publication Date: 2006 Dec |
Date Detail:
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Created Date: 2006-11-13 Completed Date: 2007-10-25 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0372525 Medline TA: Biopolymers Country: United States |
Other Details:
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Languages: eng Pagination: 584-94 Citation Subset: IM |
Copyright Information:
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Copyright 2006 Wiley Periodicals, Inc. |
Affiliation:
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Dipartimento di Scienze Farmaceutiche, Via Ponte Don Melillo, 84084 Fisciano (SA), Italy. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Base Pairing
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physiology* DNA / chemistry*, metabolism* G-Quadruplexes Oligonucleotides Oligonucleotides, Antisense / chemistry*, metabolism* Thermodynamics* |
| Chemical | |
Reg. No./Substance:
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0/Oligonucleotides; 0/Oligonucleotides, Antisense; 0/locked nucleic acid; 9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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