| Endurance training, expression, and physiology of LDH, MCT1, and MCT4 in human skeletal muscle. | |
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MedLine Citation:
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PMID: 10751188 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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To evaluate the effects of endurance training on the expression of monocarboxylate transporters (MCT) in human vastus lateralis muscle, we compared the amounts of MCT1 and MCT4 in total muscle preparations (MU) and sarcolemma-enriched (SL) and mitochondria-enriched (MI) fractions before and after training. To determine if changes in muscle lactate release and oxidation were associated with training-induced changes in MCT expression, we correlated band densities in Western blots to lactate kinetics determined in vivo. Nine weeks of leg cycle endurance training [75% peak oxygen consumption (VO(2 peak))] increased muscle citrate synthase activity (+75%, P < 0.05) and percentage of type I myosin heavy chain (+50%, P < 0.05); percentage of MU lactate dehydrogenase-5 (M4) isozyme decreased (-12%, P < 0.05). MCT1 was detected in SL and MI fractions, and MCT4 was localized to the SL. Muscle MCT1 contents were consistent among subjects both before and after training; in contrast, MCT4 contents showed large interindividual variations. MCT1 amounts significantly increased in MU, SL, and MI after training (+90%, +60%, and +78%, respectively), whereas SL but not MU MCT4 content increased after training (+47%, P < 0.05). Mitochondrial MCT1 content was negatively correlated to net leg lactate release at rest (r = -0.85, P < 0.02). Sarcolemmal MCT1 and MCT4 contents correlated positively to net leg lactate release at 5 min of exercise at 65% VO(2 peak) (r = 0.76, P < 0.03 and r = 0. 86, P < 0.01, respectively). Results support the conclusions that 1) endurance training increases expression of MCT1 in muscle because of insertion of MCT1 into both sarcolemmal and mitochondrial membranes, 2) training has variable effects on sarcolemmal MCT4, and 3) both MCT1 and MCT4 participate in the cell-cell lactate shuttle, whereas MCT1 facilitates operation of the intracellular lactate shuttle. |
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Authors:
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H Dubouchaud; G E Butterfield; E E Wolfel; B C Bergman; G A Brooks |
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Publication Detail:
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Type: Clinical Trial; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: American journal of physiology. Endocrinology and metabolism Volume: 278 ISSN: 0193-1849 ISO Abbreviation: Am. J. Physiol. Endocrinol. Metab. Publication Date: 2000 Apr |
Date Detail:
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Created Date: 2000-05-01 Completed Date: 2000-05-01 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 100901226 Medline TA: Am J Physiol Endocrinol Metab Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: E571-9 Citation Subset: IM |
Affiliation:
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Department of Integrative Biology, University of California, Berkeley 94720, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adult Amino Acid Sequence Anaerobic Threshold / physiology Blotting, Western Carrier Proteins / metabolism, physiology* Humans Isoenzymes L-Lactate Dehydrogenase / metabolism, physiology* Lactic Acid / metabolism Male Mitochondria, Muscle / enzymology, metabolism Molecular Sequence Data Monocarboxylic Acid Transporters Muscle Proteins* Muscle, Skeletal / enzymology, physiology* Myosin Heavy Chains / metabolism Oxidation-Reduction Physical Endurance / physiology* Physical Fitness / physiology* Prostaglandin-Endoperoxide Synthases / biosynthesis Subcellular Fractions / metabolism |
| Grant Support | |
ID/Acronym/Agency:
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AR-42906/AR/NIAMS NIH HHS; DK-19577/DK/NIDDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Carrier Proteins; 0/Isoenzymes; 0/Monocarboxylic Acid Transporters; 0/Muscle Proteins; 0/Myosin Heavy Chains; 0/SLC16A4 protein, human; 50-21-5/Lactic Acid; EC 1.1.1.27/L-Lactate Dehydrogenase; EC 1.14.99.1/Prostaglandin-Endoperoxide Synthases |
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