| Endotoxin reduces maximal oxygen consumption in hepatocytes independent of any hypoxic insult. | |
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MedLine Citation:
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PMID: 9722044 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: The cause of the metabolic disturbances in sepsis remains uncertain, but there is increasing evidence suggesting that haemodynamic changes are not solely responsible. We addressed the question of whether endotoxin has a significant effect on cellular oxygen metabolism, independent of confounding haemodynamic defects. DESIGN: Prospective, controlled experimental study. Setting: University Laboratory. MODEL: Human hepatocyte cell line. METHODS: The oxygen consumption rate (OCR) was calculated from the fall in oxygen tension in a sealed cuvette containing Hep G2 cells in suspension. The oxygen tension was measured by porphyrin phosphorescence half-life analysis. Resting OCR was measured in control cells and after 1, 6 and 24 h of endotoxin exposure. In a second series of experiments, resting and maximal OCR was measured after 6 and 24 h of endotoxin exposure and in control cells using the addition of a mitochondrial uncoupler (FCCP); this uncouples the respiratory chain from ATP synthesis, thereby removing negative feedback and allowing the respiratory chain to work at maximal rate. RESULTS: Endotoxin caused a rise in resting OCR at 1 h which was significant by 6 h but had returned to control values by 24 h. Maximal OCR also increased at 6 h, however exposure to endotoxin for 24 h significantly reduced maximal OCR compared to the control cells. CONCLUSIONS: Endotoxin has complex effects on cellular energy metabolism causing an initial rise in the oxygen consumption rate and a significant limitation in oxygen consumption capacity at 24 h. These complex effects would be in keeping with the varied responses seen in patients. |
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Authors:
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D M Rosser; M Manji; H Cooksley; G Bellingan |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Intensive care medicine Volume: 24 ISSN: 0342-4642 ISO Abbreviation: Intensive Care Med Publication Date: 1998 Jul |
Date Detail:
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Created Date: 1998-11-10 Completed Date: 1998-11-10 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7704851 Medline TA: Intensive Care Med Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 725-9 Citation Subset: IM; S |
Affiliation:
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Department of Anaesthesia & Intensive Care, University Hospital, University of Birmingham, Edgbaston, UK. s.m.baker@bham.ac.uk |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Anoxia
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metabolism*,
microbiology*,
physiopathology Cell Line Endotoxins / adverse effects* Hemodynamics Humans Liver / cytology, metabolism* Liver Circulation Microcirculation Oxygen Consumption* Prospective Studies Shock, Septic / metabolism*, microbiology*, physiopathology |
| Chemical | |
Reg. No./Substance:
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0/Endotoxins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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