Document Detail


Endotoxin levels in cirrhotic rats with sterile and infected ascites.
MedLine Citation:
PMID:  1624078     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Endotoxin levels were measured in sterile and bacterially infected ascites in a rat model of phenobarbital and carbon tetrachloride induced cirrhosis was used. An improved chromogenic substrate assay was used to measure endotoxin. All rat ascites specimens were positive for endotoxin. In culture-negative ascites (n = 8), it ranged from 0.05 EU/ml to 0.14 EU/ml (0.08 +/- 0.04 EU/ml, mean +/- SD) (Escherichia coli 0111:B4 endotoxin was used as a reference). In culture-positive ascites (premortem n = 3, postmortem n = 1), it ranged from 0.78 EU/ml to 1.8 EU/ml (1.29 +/- 0.59 EU/ml, mean +/- SD). All rats with premortem culture-positive ascites died within two days. This model is useful to study ascites endotoxin levels. In this study, increasing levels of ascites endotoxin correlated with spontaneous bacterial peritonitis and death.
Authors:
S Sugano
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Gastroenterologia Japonica     Volume:  27     ISSN:  0435-1339     ISO Abbreviation:  Gastroenterol. Jpn.     Publication Date:  1992 Jun 
Date Detail:
Created Date:  1992-08-12     Completed Date:  1992-08-12     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0152744     Medline TA:  Gastroenterol Jpn     Country:  JAPAN    
Other Details:
Languages:  eng     Pagination:  348-53     Citation Subset:  IM    
Affiliation:
Saiseikai Wakakusa Hospital, Yokohama, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Ascitic Fluid / chemistry*,  pathology
Chromogenic Compounds
Disease Models, Animal
Endotoxins / analysis*
Escherichia coli*
Liver Cirrhosis, Experimental* / pathology
Male
Rats
Rats, Inbred Strains
Chemical
Reg. No./Substance:
0/Chromogenic Compounds; 0/Endotoxins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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