| Endotoxin inactivating activity of rat serum. | |
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MedLine Citation:
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PMID: 3945619 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The ability of rat serum to inactivate endotoxin (LPS) was assessed with the aid of the limulus amebocyte lysate assay. Following the addition of various amounts of endotoxin to normal serum the mixture was incubated for 1 hr at 37 degrees C and the residual endotoxin activity determined. One milliliter of rat serum inactivated between 5 and 10 micrograms Escherichia coli LPS per hour. Heating serum for 45 min at 56 degrees C resulted in loss of 80-90% of the LPS inhibitor (LPSI) activity. Serum from cobra venom factor (CVF)-treated rats inactivated between 0.5 and 2.5 micrograms LPS/ml serum. Serum from tolerant rats, even after heating for 45 min at 56 degrees C, inactivates between 10 and 15 micrograms LPS/ml serum/hr; decomplemented tolerant rat serum neutralizes between 5 and 10 micrograms LPS/ml serum/hr. Clearly, the tolerant rat has large quantities of LPSI activity, which does not appear to be complement. The inhibitor found in tolerant rat serum is not species specific since it inactivates Salmonella minnesota and Salmonella typhimurium endotoxins to the same degree and in the same amount as E. coli endotoxin, the agent used to induce tolerance. Both heating serum (56 degrees C) and lead acetate reduce LPSI activity. |
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Authors:
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Y Yamaguchi; P A Billing; J L Babb; K Mori; M Akagi; H Gans |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.) Volume: 181 ISSN: 0037-9727 ISO Abbreviation: Proc. Soc. Exp. Biol. Med. Publication Date: 1986 Jan |
Date Detail:
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Created Date: 1986-02-28 Completed Date: 1986-02-28 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 7505892 Medline TA: Proc Soc Exp Biol Med Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 163-8 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Blood Physiological Phenomena* Complement System Proteins / physiology Endotoxins / antagonists & inhibitors* Hot Temperature Lead / pharmacology Lipopolysaccharides / antagonists & inhibitors* Organometallic Compounds* Rats Rats, Inbred Strains |
| Chemical | |
Reg. No./Substance:
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0/Endotoxins; 0/Lipopolysaccharides; 0/Organometallic Compounds; 301-04-2/lead acetate; 7439-92-1/Lead; 9007-36-7/Complement System Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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