Document Detail

Endotoxin in pooled pericardial blood contributes to the systemic inflammatory response during cardiac surgery.
MedLine Citation:
PMID:  11001165     Owner:  NLM     Status:  MEDLINE    
Although endotoxin has been implicated as an important contributor to the systemic inflammatory response (SIR) during cardiopulmonary bypass (CPB), its source remains unclear. While gut translocation has traditionally been perceived as the primary source of endotoxemia, accumulation of endotoxin in pooled pericardial blood may represent an additional source of endotoxin that is continually reinfused into the CPB circuit. Eighteen patients undergoing primary coronary revascularization procedures were prospectively evaluated. Shed blood pooled in the pericardial space was returned to the CPB circuit through cardiotomy suction catheters at 45 min after placement of the aortic cross-clamp. Simultaneous samples of pooled pericardial and peripheral arterial blood were obtained and analyzed by a limulus amebocyte lysate assay for the determination of endotoxin concentration, and an enzyme-linked immunosorbent assay for tumor necrosis factor (TNF-alpha) levels. Significant elevations in endotoxin were demonstrated in pooled pericardial blood samples compared with arterial blood (3.5 +/- 0.5 vs 0.8 +/- 0.2 pg/ml; p < 0.05). TNF-alpha levels were below the limits of detection in both samples. These data implicate pooled pericardial blood as an important primary source of endotoxin that, when continually reinfused throughout CPB, may contribute to the overall SIR. Because endotoxemia has been identified as an important predictor of adverse outcomes following cardiac surgery, removal of endotoxin antigen in shed pericardial blood, prior to its reinfusion into the CPB circuit, may provide a directed means to improve perioperative outcome without compromising established blood conservation techniques.
T Spanier; K Tector; G Schwartz; J Chen; M Oz; J Beck; L Mongero
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Perfusion     Volume:  15     ISSN:  0267-6591     ISO Abbreviation:  Perfusion     Publication Date:  2000 Sep 
Date Detail:
Created Date:  2000-12-26     Completed Date:  2001-03-15     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  8700166     Medline TA:  Perfusion     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  427-31     Citation Subset:  IM    
Division of Cardiothoracic Surgery and Perfusion, Columbia University, USA.
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MeSH Terms
Aged, 80 and over
Cardiopulmonary Bypass / adverse effects*
Endotoxins / blood*,  pharmacology
Enzyme-Linked Immunosorbent Assay
Equipment and Supplies / microbiology
Inflammation / chemically induced*,  etiology
Limulus Test
Middle Aged
Myocardial Revascularization / adverse effects
Pericardium / chemistry*
Prospective Studies
Tumor Necrosis Factor-alpha / metabolism
Reg. No./Substance:
0/Endotoxins; 0/Tumor Necrosis Factor-alpha

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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