Document Detail

Endothelium-dependent vasorelaxation effect of rutin-free tartary buckwheat extract in isolated rat thoracic aorta.
MedLine Citation:
PMID:  18328684     Owner:  NLM     Status:  MEDLINE    
The aim of this study was to point out the potential of tartary buckwheat on vascular functions. A nonabsorbed fraction of hot-water extract of tartary buckwheat on a SP70 column (TBSP-T), which was free from rutin, was used for this aim. In a contractile experiment using Sprague-Dawley rat thoracic aorta rings contracted by 1.0 microM phenylephrine (PE) or 50 mM KCl, TBSP-T evoked a significant vasorelaxation [EC50 (mg/ml): PE; 2.2; KCl, 1.9]. By a further fractionation of TBSP-T by liquid-liquid partitioning into basic, neutral and acidic fractions, a marked enhancement of vasorelaxation effect was observed only for acidic fraction (EC50, 0.25 mg/ml). The action of acidic fraction was significantly attenuated in endothelium-denuded aortic rings and in the presence of nitric oxide synthase inhibitor, NG-monomethyl-L-arginine (100 microM). The fraction also enhanced the cyclic guanosine monophosphate (cGMP) production in aortic rings contracted with PE [cGMP (pmol/mg protein): PE, 7.2+/-2.3; PE+Acidic fraction, 35+/-8]. These results indicate that acidic fraction could mediate NO/cGMP pathways, thereby exerting endothelium-dependent vasorelaxation action. In conclusion, tartary buckwheat was proven to regulate vascular tones and have latent acidic candidates except for rutin.
Yusuke Ushida; Toshiro Matsui; Mitsuru Tanaka; Kiyoshi Matsumoto; Hirokazu Hosoyama; Atsuhiro Mitomi; Yuko Sagesaka; Takami Kakuda
Related Documents :
4041474 - Secretion of phospholipase a1 by bone marrow-derived macrophages.
19244244 - Identification of a cardiolipin-specific phospholipase encoded by the gene cld1 (ygr110...
6401324 - Serum albumin washing specifically enhances arachidonate incorporation into synaptosoma...
1139394 - Distribution of newly formed fatty acids among glycerolipids of isolated perfused rat l...
8321354 - Effects of a protein meal on blood amino acid profile in patients with chronic renal fa...
8303714 - Effect of l-ascorbic acid pretreatment on cadmium toxicity in the male fischer (f344/nc...
Publication Detail:
Type:  In Vitro; Journal Article     Date:  2008-03-06
Journal Detail:
Title:  The Journal of nutritional biochemistry     Volume:  19     ISSN:  0955-2863     ISO Abbreviation:  J. Nutr. Biochem.     Publication Date:  2008 Oct 
Date Detail:
Created Date:  2008-09-12     Completed Date:  2008-11-12     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9010081     Medline TA:  J Nutr Biochem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  700-7     Citation Subset:  IM    
Faculty of Agriculture, Graduate School of Kyushu University, Higashi-ku, Fukuoka 812-8581 Japan.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Aorta, Thoracic / drug effects*,  physiology
Endothelium, Vascular / drug effects*,  physiology
Fagopyrum / chemistry*
Free Radical Scavengers / chemistry,  pharmacology
Plant Extracts / chemistry,  pharmacology*
Rutin / chemistry*
Vasodilation / drug effects*
Reg. No./Substance:
0/Free Radical Scavengers; 0/Plant Extracts; 153-18-4/Rutin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Does measuring BHR add to guideline derived clinical measures in determining treatment for patients ...
Next Document:  Dietary oleic acid and adipocyte lipolytic activity in culture.