| Endothelium-dependent vasorelaxation effect of rutin-free tartary buckwheat extract in isolated rat thoracic aorta. | |
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MedLine Citation:
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PMID: 18328684 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The aim of this study was to point out the potential of tartary buckwheat on vascular functions. A nonabsorbed fraction of hot-water extract of tartary buckwheat on a SP70 column (TBSP-T), which was free from rutin, was used for this aim. In a contractile experiment using Sprague-Dawley rat thoracic aorta rings contracted by 1.0 microM phenylephrine (PE) or 50 mM KCl, TBSP-T evoked a significant vasorelaxation [EC50 (mg/ml): PE; 2.2; KCl, 1.9]. By a further fractionation of TBSP-T by liquid-liquid partitioning into basic, neutral and acidic fractions, a marked enhancement of vasorelaxation effect was observed only for acidic fraction (EC50, 0.25 mg/ml). The action of acidic fraction was significantly attenuated in endothelium-denuded aortic rings and in the presence of nitric oxide synthase inhibitor, NG-monomethyl-L-arginine (100 microM). The fraction also enhanced the cyclic guanosine monophosphate (cGMP) production in aortic rings contracted with PE [cGMP (pmol/mg protein): PE, 7.2+/-2.3; PE+Acidic fraction, 35+/-8]. These results indicate that acidic fraction could mediate NO/cGMP pathways, thereby exerting endothelium-dependent vasorelaxation action. In conclusion, tartary buckwheat was proven to regulate vascular tones and have latent acidic candidates except for rutin. |
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Authors:
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Yusuke Ushida; Toshiro Matsui; Mitsuru Tanaka; Kiyoshi Matsumoto; Hirokazu Hosoyama; Atsuhiro Mitomi; Yuko Sagesaka; Takami Kakuda |
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Publication Detail:
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Type: In Vitro; Journal Article Date: 2008-03-06 |
Journal Detail:
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Title: The Journal of nutritional biochemistry Volume: 19 ISSN: 0955-2863 ISO Abbreviation: J. Nutr. Biochem. Publication Date: 2008 Oct |
Date Detail:
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Created Date: 2008-09-12 Completed Date: 2008-11-12 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9010081 Medline TA: J Nutr Biochem Country: United States |
Other Details:
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Languages: eng Pagination: 700-7 Citation Subset: IM |
Affiliation:
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Faculty of Agriculture, Graduate School of Kyushu University, Higashi-ku, Fukuoka 812-8581 Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Aorta, Thoracic / drug effects*, physiology Endothelium, Vascular / drug effects*, physiology Fagopyrum / chemistry* Free Radical Scavengers / chemistry, pharmacology Plant Extracts / chemistry, pharmacology* Rats Rutin / chemistry* Vasodilation / drug effects* |
| Chemical | |
Reg. No./Substance:
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0/Free Radical Scavengers; 0/Plant Extracts; 153-18-4/Rutin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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