Document Detail


Endothelium-dependent vasorelaxation effect of rutin-free tartary buckwheat extract in isolated rat thoracic aorta.
MedLine Citation:
PMID:  18328684     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The aim of this study was to point out the potential of tartary buckwheat on vascular functions. A nonabsorbed fraction of hot-water extract of tartary buckwheat on a SP70 column (TBSP-T), which was free from rutin, was used for this aim. In a contractile experiment using Sprague-Dawley rat thoracic aorta rings contracted by 1.0 microM phenylephrine (PE) or 50 mM KCl, TBSP-T evoked a significant vasorelaxation [EC50 (mg/ml): PE; 2.2; KCl, 1.9]. By a further fractionation of TBSP-T by liquid-liquid partitioning into basic, neutral and acidic fractions, a marked enhancement of vasorelaxation effect was observed only for acidic fraction (EC50, 0.25 mg/ml). The action of acidic fraction was significantly attenuated in endothelium-denuded aortic rings and in the presence of nitric oxide synthase inhibitor, NG-monomethyl-L-arginine (100 microM). The fraction also enhanced the cyclic guanosine monophosphate (cGMP) production in aortic rings contracted with PE [cGMP (pmol/mg protein): PE, 7.2+/-2.3; PE+Acidic fraction, 35+/-8]. These results indicate that acidic fraction could mediate NO/cGMP pathways, thereby exerting endothelium-dependent vasorelaxation action. In conclusion, tartary buckwheat was proven to regulate vascular tones and have latent acidic candidates except for rutin.
Authors:
Yusuke Ushida; Toshiro Matsui; Mitsuru Tanaka; Kiyoshi Matsumoto; Hirokazu Hosoyama; Atsuhiro Mitomi; Yuko Sagesaka; Takami Kakuda
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Publication Detail:
Type:  In Vitro; Journal Article     Date:  2008-03-06
Journal Detail:
Title:  The Journal of nutritional biochemistry     Volume:  19     ISSN:  0955-2863     ISO Abbreviation:  J. Nutr. Biochem.     Publication Date:  2008 Oct 
Date Detail:
Created Date:  2008-09-12     Completed Date:  2008-11-12     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9010081     Medline TA:  J Nutr Biochem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  700-7     Citation Subset:  IM    
Affiliation:
Faculty of Agriculture, Graduate School of Kyushu University, Higashi-ku, Fukuoka 812-8581 Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Aorta, Thoracic / drug effects*,  physiology
Endothelium, Vascular / drug effects*,  physiology
Fagopyrum / chemistry*
Free Radical Scavengers / chemistry,  pharmacology
Plant Extracts / chemistry,  pharmacology*
Rats
Rutin / chemistry*
Vasodilation / drug effects*
Chemical
Reg. No./Substance:
0/Free Radical Scavengers; 0/Plant Extracts; 153-18-4/Rutin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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