Document Detail

Endothelium-dependent inhibitory effects of acetylcholine, adenosine triphosphate, thrombin and arachidonic acid in the canine femoral artery.
MedLine Citation:
PMID:  6806467     Owner:  NLM     Status:  MEDLINE    
Experiments were designed to investigate the importance of endothelial metabolism of arachidonic acid in the relaxation of isolated arteries caused by acetylcholine, ATP, arachidonic acid itself and thrombin. Rings of canine femoral arteries were mounted for isometric tension recording in organ chambers filled with Krebs-Ringer-bicarbonate solution. Acetylcholine, arachidonic acid, ATP, 15-hydroperoxy-5,8,11,13 eicosatetraenoic acid, thrombin and prostacyclin caused relaxations of control rings made to contract with norepinephrine. Removal of the endothelium abolished the relaxations caused by acetylcholine, ATP, thrombin and 15-hydroperoxy-5,8,11,13 eicosatetraenoic acid, reduced those caused by arachidonic acid, but did not affect the inhibitory effect of prostacyclin. The inhibitory response to arachidonic acid was abolished by indomethacin and 5,8,11,14-eicosatetraynoic acid (ETYA); that to acetylcholine was abolished by mepacrine and reduced by ETYA. The relaxations induced by thrombin and ATP were not affected by these inhibitors. Canine femoral arteries with endothelium, but not de-endothelialized preparations, transformed part of exogenously added [14C] arachidonic acid to prostaglandins (6-oxo-prostaglandin (F1 alpha) and a hydroxy derivative. The formation of prostanoids was inhibited by indomethacin, ETYA and 15-hydroperoxy-5, 8,11,13 eicosatetraenoic acid and that of hydroxy derivative by ETYA. These results suggest that the endothelial cells of canine femoral arteries initiate relaxation of the vascular smooth muscle cells of the media by: 1) producing prostacyclin, when exposed to arachidonic acid; 2) producing a lipoxygenase product, when exposed to acetylcholine; and 3) producing a signal of unknown nature, when exposed to thrombin or ATP.
J G De Mey; M Claeys; P M Vanhoutte
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of pharmacology and experimental therapeutics     Volume:  222     ISSN:  0022-3565     ISO Abbreviation:  J. Pharmacol. Exp. Ther.     Publication Date:  1982 Jul 
Date Detail:
Created Date:  1982-08-26     Completed Date:  1982-08-26     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0376362     Medline TA:  J Pharmacol Exp Ther     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  166-73     Citation Subset:  IM    
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MeSH Terms
Acetylcholine / pharmacology*
Adenosine Triphosphate / pharmacology*
Arachidonic Acid
Arachidonic Acids / pharmacology*
Cells, Cultured
Endothelium / cytology,  physiology
Femoral Artery / drug effects
Muscle Contraction / drug effects
Muscle, Smooth, Vascular / drug effects*
Thrombin / pharmacology*
Grant Support
Reg. No./Substance:
0/Arachidonic Acids; 506-32-1/Arachidonic Acid; 51-84-3/Acetylcholine; 56-65-5/Adenosine Triphosphate; EC

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