Document Detail

Endothelium-dependent and -independent vasodilator effects of eugenol in the rat mesenteric vascular bed.
MedLine Citation:
PMID:  12724042     Owner:  NLM     Status:  MEDLINE    
The possible involvement of the endothelium in the vasodilator action of eugenol was investigated in the mesenteric vascular bed (MVB) of the rat. Bolus injections of eugenol (0.2, 2 and 20 micromol) and acetylcholine (ACh; 10, 30 and 100 pmol) induced dose-dependent vasodilator responses in noradrenaline-precontracted beds that were partially inhibited by pretreatment of the MVB with deoxycholate (1 mg mL(-1)) to remove the endothelium (approximately 14% and approximately 30% of the control response remaining at the lowest doses of ACh and eugenol, respectively). The vasodilator effect of glyceryl trinitrate (1 micromol) was unaltered by deoxycholate. In the presence of either N(omega)-nitro-L-arginine methyl ester (300 microM) or tetraethylammonium (1 mM)the response to ACh was partially reduced, whereas eugenol-induced vasodilation was unaffected. Similarly the vasodilator effect of eugenol was not inhibited by indometacin (3 microM). Under calcium-free conditions the vasoconstrictor response elicited by bolus injections of noradrenaline (10 nmol) was dose-dependently and completely inhibited by eugenol (0.1-1 mM). Additionally, the pressor effects of bolus injections of calcium chloride in potassium-depolarized MVBs were greatly reduced in the presence of eugenol (0.1 mM), with a maximal reduction of approximately 71% of the control response. Our data showed that eugenol induced dose-dependent, reversible vasodilator responses in the rat MVB, that were partially dependent on the endothelium, although apparently independent of nitric oxide, endothelium-derived hyperpolarizing factor or prostacyclin. Furthermore, an endothelium-independent intracellular site of action seemed likely to participate in its smooth muscle relaxant properties.
David Neil Criddle; Socorro Vanesca Frota Madeira; Roberto Soares de Moura
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of pharmacy and pharmacology     Volume:  55     ISSN:  0022-3573     ISO Abbreviation:  J. Pharm. Pharmacol.     Publication Date:  2003 Mar 
Date Detail:
Created Date:  2003-05-01     Completed Date:  2003-07-08     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0376363     Medline TA:  J Pharm Pharmacol     Country:  England    
Other Details:
Languages:  eng     Pagination:  359-65     Citation Subset:  IM    
Laboratório de Farmacologia dos Canais Iônicos, Departamento de Ciências Fisiológicas, CCS, Universidade Estadual do Ceará, Av. Paranjana 1700, Fortaleza CE 60740-000, Brazil.
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MeSH Terms
Acetylcholine / pharmacology
Anti-Inflammatory Agents, Non-Steroidal / pharmacology
Blood Pressure / drug effects
Calcium / physiology
Deoxycholic Acid / pharmacology
Endothelium, Vascular / physiology*
Enzyme Inhibitors / pharmacology
Eugenol / pharmacology*
Indomethacin / pharmacology
NG-Nitroarginine Methyl Ester / pharmacology
Nitric Oxide Synthase / antagonists & inhibitors
Nitric Oxide Synthase Type III
Norepinephrine / pharmacology
Potassium Channel Blockers / pharmacology
Rats, Wistar
Splanchnic Circulation / drug effects*
Tetraethylammonium / pharmacology
Trinitrotoluene / pharmacology
Vasoconstrictor Agents / pharmacology
Vasodilation / drug effects*
Vasodilator Agents / pharmacology
Reg. No./Substance:
0/Anti-Inflammatory Agents, Non-Steroidal; 0/Enzyme Inhibitors; 0/Potassium Channel Blockers; 0/Vasoconstrictor Agents; 0/Vasodilator Agents; 118-96-7/Trinitrotoluene; 50903-99-6/NG-Nitroarginine Methyl Ester; 51-41-2/Norepinephrine; 51-84-3/Acetylcholine; 53-86-1/Indomethacin; 66-40-0/Tetraethylammonium; 7440-70-2/Calcium; 83-44-3/Deoxycholic Acid; 97-53-0/Eugenol; EC Oxide Synthase; EC Oxide Synthase Type III; EC protein, rat

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