| Endothelin-like immunoreactivity in lactotrophs, gonadotrophs, and somatotrophs of rat anterior pituitary gland are affected differentially by ovarian steroid hormones. | |
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MedLine Citation:
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PMID: 11394645 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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It has been previously found that all hormone-producing phenotypes of the anterior lobe of the pituitary gland are capable of producing endothelin (ET)-like substances. The aim of this study was to determine whether the expression of ET-1-like peptides in lactotrophs, gonadotrophs, and somatotrophs is influenced by different in vivo ovarian hormonal conditions. Anterior lobes of the pituitary gland were harvested from ovariectomized and ovarian steroid-replaced adult female rats 10-12 d after surgery. Quantitative immunocytochemistry was performed on enzymatically dispersed pituitary cells. The presence of ET-1-like immunoreactivity in prolactin-, luteinizing hormone-, or growth hormone-producing cells was demonstrated by double-label immunocytochemistry. The incidence of ET-1 immunopositive pituitary cells was unaffected by progesterone treatment alone. Estradiol replacement caused a modest decrease in the number of lactotrophs and somatotrophs expressing ET-1 but increased the incidence of ET-1 immunopositive cells among gonadotrophs. Combined treatment with estradiol and progesterone robustly increased the incidence of ET-1 immunopositive lactotrophs and gonadotrophs but had no effect on somatotrophs. These data reveal that the synthesis of ET-1-like peptides in lactotrophs and gonadotrophs (and, to a lesser extent, in somatotrophs) is sensitive to ovarian steroids. Furthermore, these findings predict that ovarian steroids modulate ET-1 biosynthesis during the estrous cycle, suggesting a possible mechanism by which the ovarian steroid milieu may regulate the responsiveness of lactotrophs and gonadotrophs to their hypothalamic secretagogues. |
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Authors:
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B Kanyicska; A Lerant; M E Freeman |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Endocrine Volume: 14 ISSN: 1355-008X ISO Abbreviation: Endocrine Publication Date: 2001 Mar |
Date Detail:
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Created Date: 2001-06-07 Completed Date: 2001-10-11 Revised Date: 2010-06-24 |
Medline Journal Info:
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Nlm Unique ID: 9434444 Medline TA: Endocrine Country: United States |
Other Details:
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Languages: eng Pagination: 263-8 Citation Subset: IM |
Affiliation:
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Department of Biological Science, Florida State University, Tallahassee 32306-4340, USA. bela@neuro.fsu.edu |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Drug Implants Endothelin-1 / analysis* Estradiol / administration & dosage, pharmacology* Female Fluorescent Antibody Technique Follicle Stimulating Hormone / analysis Growth Hormone / analysis* Immunohistochemistry Luteinizing Hormone / analysis Ovariectomy Pituitary Gland, Anterior / chemistry*, drug effects, metabolism Progesterone / administration & dosage, pharmacology* Prolactin / analysis* Rats Rats, Sprague-Dawley |
| Grant Support | |
ID/Acronym/Agency:
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HD-38551/HD/NICHD NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Drug Implants; 0/Endothelin-1; 50-28-2/Estradiol; 57-83-0/Progesterone; 9002-62-4/Prolactin; 9002-67-9/Luteinizing Hormone; 9002-68-0/Follicle Stimulating Hormone; 9002-72-6/Growth Hormone |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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