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Endothelin-1 stimulates human trophoblast cell migration through Cdc42 activation.
MedLine Citation:
PMID:  22770822     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
PURPOSE: This study investigated the role and mechanism of Cdc42 in Endothelin-1 (ET-1)-induced trophoblast cell migration. METHODS: We examined ET-1-mediated stimulation of trophoblast migration with HTR-8/SVneo cells. Cdc42 activation was measured after ET-1 treatment of HTR-8/SVneo cells. To determine the ET receptor subtype involved in ET-1-mediated Cdc42 activation, experiments were performed in the presence of ET(A) and ET(B) receptor antagonists. Finally, using siRNA we knocked down the expression of Cdc42 to examine the involvement of Cdc42 in the regulation of ET-1-stimulated trophoblast cell migration. RESULTS: ET-1 was shown to have a dose-dependent effect on trophoblast migration. At low concentrations of ET-1 (0.1 nmol/L) ET-1 had a stimulatory effect on cell migration. ET-1 (10 nmol/L) increased HTR-8/svneo cell migration index by 2.5 fold. ET-1 (10 nmol/L) elevated protein level and activity of Cdc42. ET-1 induced activation of Cdc42 GTPase was mediated by both ET(A) and ET(B). ET-1-induced cell migration was shown to be inhibited by Cdc42 siRNA.The inhibition was not mitigated by the addition of ET-1, suggesting that Cdc42 plays an important role in trophoblast migration and is obligatory for ET-1 action. CONCLUSIONS: ET-1 stimulates EVT migration through Cdc42 activation.
Authors:
S Liu; Q Li; Q Na; C Liu
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-7-6
Journal Detail:
Title:  Placenta     Volume:  -     ISSN:  1532-3102     ISO Abbreviation:  -     Publication Date:  2012 Jul 
Date Detail:
Created Date:  2012-7-9     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8006349     Medline TA:  Placenta     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012 Elsevier Ltd. All rights reserved.
Affiliation:
Department of Obstetrics and Gynecology, Shengjing Hospital of China Medical University, Shenyang, Liaoning 110004, PR China.
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