Document Detail

Endothelin-1 regulates rat bone sialoprotein gene transcription.
MedLine Citation:
PMID:  20587945     Owner:  NLM     Status:  MEDLINE    
Endothelin-1 (ET-1) was originally discovered as a vasoconstrictor protein excreted by vascular endothelial cells. Recently, tumor-produced ET-1 has been considered to stimulate osteoblasts to form new bone, and to be an important mediator of osteoblastic bone metastasis. ET-1 has high affinity for two different membrane receptors, ET(A)R and ET(B)R, which are expressed by many types of cells including osteoblasts. Bone sialoprotein (BSP) is a phosphorylated and sulfated glycoprotein associated with mineralized connective tissues. To investigate the effects of ET-1 on BSP transcription, we used rat osteoblast-like ROS17/2.8 cells. Levels of BSP and osteopontin mRNA were increased at 12 h after treatment with ET-1 (10 ng/ml), and ET-1 at the same concentration induced luciferase activity of a -116 to +60 BSP promoter construct at 6 h. Transcriptional activity of -84BSPLUC, which contains the cAMP response element (CRE), was increased by ET-1. Furthermore, at 6 h, ET-1 (10 ng/ml) increased the binding of nuclear protein to CRE, the FGF2 response element (FRE) and the homeodomain protein-binding site (HOX). Antibodies against CREB1, JunD and Fra2 disrupted the formation of CRE-protein complexes, while antibodies against Runx2 and Dlx5 reduced the formation of FRE- and HOX-protein complexes. These findings indicate that ET-1 increases BSP transcription via the CRE, FRE and HOX sites in the rat BSP gene promoter.
Xinyue Li; Zhitao Wang; Li Yang; Zhengyang Li; Yorimasa Ogata
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of oral science     Volume:  52     ISSN:  1880-4926     ISO Abbreviation:  J Oral Sci     Publication Date:  2010 Jun 
Date Detail:
Created Date:  2010-06-30     Completed Date:  2010-09-30     Revised Date:  2011-11-03    
Medline Journal Info:
Nlm Unique ID:  9808942     Medline TA:  J Oral Sci     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  221-9     Citation Subset:  D; IM    
Department of Periodontology, Nihon University School of Dentistry at Matsudo, Chiba, Japan.
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MeSH Terms
Cell Line, Tumor
Core Binding Factor Alpha 1 Subunit / antagonists & inhibitors
Cyclic AMP / genetics
Cyclic AMP Response Element-Binding Protein / antagonists & inhibitors,  genetics
Endothelin-1 / genetics*
Fibroblast Growth Factor 2 / genetics
Fos-Related Antigen-2 / antagonists & inhibitors
Gene Expression Regulation / genetics
Homeodomain Proteins / antagonists & inhibitors,  genetics
Integrin-Binding Sialoprotein
Osteoblasts / metabolism
Osteopontin / analysis,  genetics
Promoter Regions, Genetic / genetics
RNA, Messenger / analysis
Response Elements / genetics
Sialoglycoproteins / analysis,  genetics*
Time Factors
Transcription Factors / antagonists & inhibitors
Transcription, Genetic / genetics*
Reg. No./Substance:
0/CREB1 protein, rat; 0/Core Binding Factor Alpha 1 Subunit; 0/Cyclic AMP Response Element-Binding Protein; 0/Distal-less homeobox proteins; 0/Endothelin-1; 0/Fos-Related Antigen-2; 0/Fosl2 protein, rat; 0/Homeodomain Proteins; 0/Ibsp protein, rat; 0/Integrin-Binding Sialoprotein; 0/JunD protein, rat; 0/RNA, Messenger; 0/Runx2 protein, rat; 0/Sialoglycoproteins; 0/Spp1 protein, rat; 0/Transcription Factors; 103107-01-3/Fibroblast Growth Factor 2; 106441-73-0/Osteopontin; 60-92-4/Cyclic AMP

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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