| Endothelin-1 promotes MMP-13 production and migration in human chondrosarcoma cells through FAK/PI3K/Akt/mTOR pathways. | |
| | |
MedLine Citation:
|
PMID: 21959927 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
Tumor malignancy is associated with several cellular properties including proliferation and ability to metastasize. Endothelin-1 (ET-1) the most potent vasoconstrictor plays a crucial role in migration and metastasis of human cancer cells. We found that treatment of human chondrosarcoma (JJ012 cells) with ET-1 increased migration and expression of matrix metalloproteinase (MMP)-13. ET-1-mediated cell migration and MMP-13 expression were reduced by pretreatment with inhibitors of focal adhesion kinase (FAK), phosphatidylinositol 3-kinase (PI3K), Akt, and mammalian target of rapamycin (mTOR), as well as the NF-κB inhibitor and the IκB protease inhibitor. In addition, ET-1 treatment induced phosphorylation of FAK, PI3K, AKT, and mTOR, and resulted in increased NF-κB-luciferase activity that was inhibited by a specific inhibitor of PI3K, Akt, mTOR, and NF-κB cascades. Taken together, these results suggest that ET-1 activated FAK/PI3K/AKT/mTOR, which in turn activated IKKα/β and NF-κB, resulting in increased MMP-13 expression and migration in human chondrosarcoma cells. J. Cell. Physiol. © 2011 Wiley Periodicals, Inc. |
| | |
Authors:
|
Min Huan Wu; Jeng-Fan Lo; Chia-Hua Kuo; James A Lin; Yueh-Min Lin; Li-Mien Chen; Fuu-Jen Tsai; Chang-Hai Tsai; Chih-Yang Huang; Chih-Hsin Tang |
Related Documents
:
|
23233447 - A high-throughput assay for phosphoprotein-specific phosphatase activity in cellular ex... 15569247 - Tyrosine hydroxylase phosphorylation: regulation and consequences. 18850267 - Short-term effects of endothelins on tyrosine hydroxylase activity and expression in th... 16722237 - Regulation of the dopamine transporter by phosphorylation. 19723537 - Protective effect of histamine h2 receptor antagonist ranitidine against rotenone-induc... 18228247 - Tlr7 and cd40 cooperate in il-6 production via enhanced jnk and ap-1 activation. |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2011-9-29 |
Journal Detail:
|
Title: Journal of cellular physiology Volume: - ISSN: 1097-4652 ISO Abbreviation: - Publication Date: 2011 Sep |
Date Detail:
|
Created Date: 2011-9-30 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0050222 Medline TA: J Cell Physiol Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
|
Copyright © 2011 Wiley Periodicals, Inc. |
Affiliation:
|
Graduate Institute of Basic Medical Science, China Medical University and Hospital, Taichung, Taiwan. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Cardiac motion in diffusion-weighted MRI of the liver: artifact and a method of correction.
Next Document: Value of Visual Inspection, Bimanual Palpation, and Intraoperative Ultrasonography During Hepatic Re...