| Endothelin-1 and pancreatic islet vasculature: studies in vivo and on isolated, vascularly perfused pancreatic islets. | |
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MedLine Citation:
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PMID: 17284574 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Endothelin-1 (ET-1) is a potent endothelium-derived vasoconstrictor, which also stimulates insulin release. The aim of the present study was to evaluate whether exogenously administered ET-1 affected pancreatic islet blood flow in vivo in rats and the islet arteriolar reactivity in vitro in mice. Furthermore, we aimed to determine the ET-receptor subtype that was involved in such responses. When applying a microsphere technique for measurements of islet blood perfusion in vivo, we found that ET-1 (5 nmol/kg) consistently and markedly decreased total pancreatic and especially islet blood flow, despite having only minor effects on blood pressure. Neither endothelin A (ET(A)) receptor (BQ-123) nor endothelin-B (ET(B)) receptor (BQ-788) antagonists, alone or in combination, could prevent this reduction in blood flow. To avoid confounding interactions in vivo, we also examined the arteriolar vascular reactivity in isolated, perfused mouse islets. In the latter preparation, we demonstrated a dose-dependent constriction in response to ET-1. Administration of BQ-123 prevented this, whereas BQ-788 induced a right shift in the response. In conclusion, the pancreatic islet vasculature is highly sensitive to exogenous ET-1, which mediates its effect mainly through ET(A) receptors. |
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Authors:
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En Yin Lai; A Erik G Persson; Birgitta Bodin; Orjan Källskog; Arne Andersson; Ulrika Pettersson; Peter Hansell; Leif Jansson |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't Date: 2007-02-06 |
Journal Detail:
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Title: American journal of physiology. Endocrinology and metabolism Volume: 292 ISSN: 0193-1849 ISO Abbreviation: Am. J. Physiol. Endocrinol. Metab. Publication Date: 2007 Jun |
Date Detail:
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Created Date: 2007-06-05 Completed Date: 2007-07-30 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100901226 Medline TA: Am J Physiol Endocrinol Metab Country: United States |
Other Details:
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Languages: eng Pagination: E1616-23 Citation Subset: IM |
Affiliation:
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Department of Medical Cell Biology, Uppsala University, Uppsala, Sweden. Enyin.Lai@mcb.uu.se |
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| MeSH Terms | |
Descriptor/Qualifier:
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Adrenal Glands
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blood supply Animals Arterioles / physiology Blood Glucose / metabolism Blood Pressure / drug effects Colon / blood supply Drug Synergism Duodenum / blood supply Endothelin-1 / pharmacology* Insulin / blood Islets of Langerhans / blood supply* Male Mice Mice, Inbred C57BL Oligopeptides / pharmacology Pancreas / blood supply Peptides, Cyclic / pharmacology Piperidines / pharmacology Rats Rats, Sprague-Dawley Receptor, Endothelin A / antagonists & inhibitors Receptor, Endothelin B / antagonists & inhibitors Regional Blood Flow / drug effects Vasoconstriction |
| Chemical | |
Reg. No./Substance:
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0/BQ 788; 0/Blood Glucose; 0/Endothelin-1; 0/Oligopeptides; 0/Peptides, Cyclic; 0/Piperidines; 0/Receptor, Endothelin A; 0/Receptor, Endothelin B; 11061-68-0/Insulin; 136553-81-6/cyclo(Trp-Asp-Pro-Val-Leu) |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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