Document Detail


Endothelin-1 and pancreatic islet vasculature: studies in vivo and on isolated, vascularly perfused pancreatic islets.
MedLine Citation:
PMID:  17284574     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Endothelin-1 (ET-1) is a potent endothelium-derived vasoconstrictor, which also stimulates insulin release. The aim of the present study was to evaluate whether exogenously administered ET-1 affected pancreatic islet blood flow in vivo in rats and the islet arteriolar reactivity in vitro in mice. Furthermore, we aimed to determine the ET-receptor subtype that was involved in such responses. When applying a microsphere technique for measurements of islet blood perfusion in vivo, we found that ET-1 (5 nmol/kg) consistently and markedly decreased total pancreatic and especially islet blood flow, despite having only minor effects on blood pressure. Neither endothelin A (ET(A)) receptor (BQ-123) nor endothelin-B (ET(B)) receptor (BQ-788) antagonists, alone or in combination, could prevent this reduction in blood flow. To avoid confounding interactions in vivo, we also examined the arteriolar vascular reactivity in isolated, perfused mouse islets. In the latter preparation, we demonstrated a dose-dependent constriction in response to ET-1. Administration of BQ-123 prevented this, whereas BQ-788 induced a right shift in the response. In conclusion, the pancreatic islet vasculature is highly sensitive to exogenous ET-1, which mediates its effect mainly through ET(A) receptors.
Authors:
En Yin Lai; A Erik G Persson; Birgitta Bodin; Orjan Källskog; Arne Andersson; Ulrika Pettersson; Peter Hansell; Leif Jansson
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-02-06
Journal Detail:
Title:  American journal of physiology. Endocrinology and metabolism     Volume:  292     ISSN:  0193-1849     ISO Abbreviation:  Am. J. Physiol. Endocrinol. Metab.     Publication Date:  2007 Jun 
Date Detail:
Created Date:  2007-06-05     Completed Date:  2007-07-30     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100901226     Medline TA:  Am J Physiol Endocrinol Metab     Country:  United States    
Other Details:
Languages:  eng     Pagination:  E1616-23     Citation Subset:  IM    
Affiliation:
Department of Medical Cell Biology, Uppsala University, Uppsala, Sweden. Enyin.Lai@mcb.uu.se
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MeSH Terms
Descriptor/Qualifier:
Adrenal Glands / blood supply
Animals
Arterioles / physiology
Blood Glucose / metabolism
Blood Pressure / drug effects
Colon / blood supply
Drug Synergism
Duodenum / blood supply
Endothelin-1 / pharmacology*
Insulin / blood
Islets of Langerhans / blood supply*
Male
Mice
Mice, Inbred C57BL
Oligopeptides / pharmacology
Pancreas / blood supply
Peptides, Cyclic / pharmacology
Piperidines / pharmacology
Rats
Rats, Sprague-Dawley
Receptor, Endothelin A / antagonists & inhibitors
Receptor, Endothelin B / antagonists & inhibitors
Regional Blood Flow / drug effects
Vasoconstriction
Chemical
Reg. No./Substance:
0/BQ 788; 0/Blood Glucose; 0/Endothelin-1; 0/Oligopeptides; 0/Peptides, Cyclic; 0/Piperidines; 0/Receptor, Endothelin A; 0/Receptor, Endothelin B; 11061-68-0/Insulin; 136553-81-6/cyclo(Trp-Asp-Pro-Val-Leu)

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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