| Endothelin-1 induces stimulation of prostaglandin synthesis in cells obtained from canine airways by bronchoalveolar lavage. | |
| | |
MedLine Citation:
|
PMID: 1584994 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Endothelin-1 (ET-1), a potent mediator released by airway epithelial cells, often exerts its effects in the lung through stimulation of arachidonic acid (AA) metabolism. To investigate its range of influence, we studied the action of ET-1 on the synthesis and release of thromboxane (TX)B2, prostaglandin (PG)D2, and histamine from canine airway cells obtained by bronchoalveolar lavage (BAL). ET-1 (10(-10), 10(-9) and 10(-8)M) stimulated production of TXB2 and PGD2 by BAL cell preparations in a dose-related manner in the absence of measurable histamine release. Release of TXB2 was 10-fold higher than that of PGD2. The effect of ET-1 on AA metabolism in alveolar macrophages was evaluated in preparations of purified (greater than 99%) cells labelled for 20-22 hrs with 3H-AA prior to stimulation. ET-1 (10(-8), 10(-7), 10(-6)M) induced significant, dose-related release of 3H-AA and its metabolites from alveolar macrophages, to levels 350% above control. These studies indicate that low levels of ET-1 can stimulate AA metabolism in resident luminal airway cells, including alveolar macrophages, and suggest that the function of these luminal cells may be modulated by the epithelium, in vivo, through the release of this peptide into the airways. |
| | |
Authors:
|
H Ninomiya; X Y Yu; S Hasegawa; E W Spannhake |
Related Documents
:
|
15633204 - Interaction of an artificial surfactant in human pulmonary epithelial cells. 199344 - The histogenesis and development of pulmonary tumorlets. 2900884 - Increased intracellular levels of calcitonin gene-related peptide-like immunoreactivity... 20087654 - Inhibitory effects of norcantharidin against human lung cancer cell growth and migration. 10336074 - Regulation of a novel pathway for cell death by lysosomal aspartic and cysteine protein... 1646284 - Role of viral ribonucleotide reductase in the increase of dttp pool size in herpes simp... |
Publication Detail:
|
Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
|
Title: Prostaglandins Volume: 43 ISSN: 0090-6980 ISO Abbreviation: Prostaglandins Publication Date: 1992 May |
Date Detail:
|
Created Date: 1992-06-18 Completed Date: 1992-06-18 Revised Date: 2007-11-14 |
Medline Journal Info:
|
Nlm Unique ID: 0320271 Medline TA: Prostaglandins Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 401-11 Citation Subset: IM |
Affiliation:
|
Department of Environmental Health Sciences, Johns Hopkins School of Hygiene and Public Health, Baltimore, Maryland 21205. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Arachidonic Acid / metabolism Bronchoalveolar Lavage Fluid / cytology* Dogs Endothelins / pharmacology* Histamine / biosynthesis Macrophages, Alveolar / drug effects, metabolism Male Prostaglandin D2 / biosynthesis* Thromboxane A2 / biosynthesis* |
| Grant Support | |
ID/Acronym/Agency:
|
HL 30195/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Endothelins; 41598-07-6/Prostaglandin D2; 506-32-1/Arachidonic Acid; 51-45-6/Histamine; 57576-52-0/Thromboxane A2 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: The distribution of intermediate filaments in intracranial vessels in humans
Next Document: The effects of mechanical stretching on fetal rat lung cell prostacyclin production.