Document Detail

Endothelin-1 in citric acid aerosol inhalation-induced airway constriction of guinea pigs.
MedLine Citation:
PMID:  12005349     Owner:  NLM     Status:  MEDLINE    
Endotheline-1 (ET-1) has been shown to enhance tachykinin-induced airway constriction. This study was designed to test whether ET-1 is involved in citric acid-induced bronchoconstriction. Forty-eight anesthetized-paralyzed guinea pigs were divided into six groups of 8 animals each: saline control; citric acid; ET-1; ET-1 + citric acid; BQ123 + ET-1 + citric acid; and BQ788 + ET-1 + citric acid. BQ123 and BQ788 are specific ETA and ETB receptor antagonists, respectively. Each animal in the saline control group received 50 breaths of 4 ml saline aerosol and in all citric acid-treated groups was given 50 breaths of 4 ml aerosol generated from 0.6 M citric acid. In all ET-1-treated groups, each animal was exposed to aerosol generated from 10(-8) M ET-1. The animal in the ET-1 + citric acid group was exposed to ET-1 5 min prior to the citric acid. For the last two groups, each animal was first exposed to aerosol generated from either 10(-5) M BQ123 or 10(-5) M BQ788. Five min later, the animal was exposed to ET-1; and then 5 min later was followed by citric acid. Dynamic respiratory compliance (Crs), forced expiratory volume in 0.1 sec (FEV(0.1)), and maximal expiratory flow at 30% total lung capacity (Vmax 30) were obtained before and 3-15 min after citric acid. Either citric acid or ET-1 inhalation caused significant decreases in Crs, FEV(0.1), and Vmax 30, indicating airway constriction. Citric acid-induced airway constriction, for most cases, was not significantly augmented by ET-1. However, either BQ123 or BQ 788 significantly attenuated the airway constriction induced by the combination of ET-1 and citric acid. Also, in an additional study, either BQ123 or BQ788 significantly attenuated citric acid-induced airway constriction. These data suggest that endogenous ET-1 plays an important role in citric acid aerosol-induced airway constriction in guinea pigs.
S C Lee; Y L Lai
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Chinese journal of physiology     Volume:  45     ISSN:  0304-4920     ISO Abbreviation:  Chin J Physiol     Publication Date:  2002 Mar 
Date Detail:
Created Date:  2002-05-13     Completed Date:  2002-10-24     Revised Date:  2009-08-12    
Medline Journal Info:
Nlm Unique ID:  7804502     Medline TA:  Chin J Physiol     Country:  China (Republic: 1949- )    
Other Details:
Languages:  eng     Pagination:  25-31     Citation Subset:  IM    
Department of Physiology, College of Medicine, National Taiwan University, Taipei, ROC.
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MeSH Terms
Administration, Inhalation
Bronchoconstriction / drug effects*
Citric Acid / pharmacology*
Endothelin-1 / physiology*
Guinea Pigs
Oligopeptides / pharmacology
Peptides, Cyclic / pharmacology
Piperidines / pharmacology
Reg. No./Substance:
0/Aerosols; 0/BQ 788; 0/Endothelin-1; 0/Oligopeptides; 0/Peptides, Cyclic; 0/Piperidines; 136553-81-6/cyclo(Trp-Asp-Pro-Val-Leu); 77-92-9/Citric Acid

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