Document Detail


Endothelial regeneration. IX. Arterial injury followed by rapid endothelial repair induces smooth-muscle-cell proliferation but not intimal thickening.
MedLine Citation:
PMID:  3425687     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Rat thoracic aortas were denuded of endothelial cells with a fine nylon filament, which removed a row of endothelium approximately 100 mu in width and caused platelets to adhere to the exposed subendothelium. A further group of rats was subjected to a sham operation where only the abdominal aorta was injured, and another group of rats was used as controls. Each animal was continuously labeled with 3H-thymidine (6.7 Ci/mM, 10 microCi/hr) for 7 days, at which time they were killed and the aortas perfusion-fixed with aldehydes. Sections of each aorta were processed for autoradiography. The thoracic aortas from animals subjected to the nylon filament injury showed no intimal thickening, but there was a significant increase in the thymidine index of the medial smooth muscle cells (SMCs) as compared with aortas from sham operation or control animals (1.02% +/- 0.44% versus 0.19% +/- 0.13% versus 0.15% +/- 0.12%). This experiment showed that continuous administration of 3H-thymidine permitted the detection of a small but significant increase in replication rates that could not be detected by the standard bolus administration of 3H-thymidine, and that selected loss of endothelium with minimal trauma to the vessel wall caused SMC proliferation without intimal thickening. These findings suggest that platelets may indeed provide SMC proliferation, but that migration of these cells into the intima may be controlled by different factor(s).
Authors:
T Tada; M A Reidy
Related Documents :
18441467 - Effectiveness of statin-eluting stent on early inflammatory response and neointimal thi...
24309907 - Lethal herpesvirosis in 16 captive horned vipers (vipera ammodytes ammodytes): patholog...
18441207 - Estradiol accelerates endothelial healing through the retrograde commitment of uninjure...
155387 - Early changes in the arterial endothelium under various pathological conditions: an ele...
1509977 - In vitro effects of paf on venous endothelial cell actin disposition.
23615837 - A case of degos disease: demonstration of c5b-9-mediated vascular injury.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The American journal of pathology     Volume:  129     ISSN:  0002-9440     ISO Abbreviation:  Am. J. Pathol.     Publication Date:  1987 Dec 
Date Detail:
Created Date:  1988-01-25     Completed Date:  1988-01-25     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0370502     Medline TA:  Am J Pathol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  429-33     Citation Subset:  AIM; IM    
Affiliation:
Department of Pathology, University of Washington, Seattle 98195.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Aorta, Thoracic / cytology,  physiology,  ultrastructure
Cell Division
Endothelium, Vascular / cytology,  physiology*
Male
Microscopy, Electron
Microscopy, Electron, Scanning
Muscle, Smooth, Vascular / cytology*
Rats
Rats, Inbred Strains
Regeneration*
Grant Support
ID/Acronym/Agency:
HL-03174/HL/NHLBI NIH HHS; HL-30203/HL/NHLBI NIH HHS
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Psychophysiological stress, elevated intraocular pressure, and acute closed-angle glaucoma.
Next Document:  Quantitative analysis of myocardial infarction in (NZW x BXSB)F1 hybrid mice with systemic lupus ery...