Document Detail


Endothelial protective effect of stobadine on ischaemia/reperfusion-induced injury.
MedLine Citation:
PMID:  9834846     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The aim of the present study was to evaluate the influence of the antioxidant stobadine on changes in the reactivity of the rat abdominal aorta induced by ischaemia and reperfusion (I/R). In anaesthetized male rats, in vivo ischaemia was elicited by occlusion of the abdominal aorta for 18 hours; reperfusion lasted 30 minutes. The aortal rings were taken from the reperfused portion. Decreased relaxant response to acetylcholine, as a consequence of endothelial injury, was seen after I/R. We also demonstrated I/R-induced reversible ultrastructural changes both in endothelial and smooth muscle cells, predominantly in the mitochondria. Lipid peroxidation was increased in homogenates of I/R aortae; the concentration of thiobarbituric acid reactive substances (TBARS) increased from a control value of 0.97 +/- 0.03 to 2.57 +/- 0.06 nmol/l/mg protein. Stobadine (2 mg/kg i.v., 5 minutes before starting reperfusion) protected the abdominal aorta against I/R-induced decrease of acetylcholine relaxation, and prevented changes in mitochondria and an increase of TBARS concentration. The protective effect of stobadine seems to be due to its antioxidant properties.
Authors:
R Sotníková; L Okruhlicová; P Noskovic
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  General physiology and biophysics     Volume:  17     ISSN:  0231-5882     ISO Abbreviation:  Gen. Physiol. Biophys.     Publication Date:  1998 Sep 
Date Detail:
Created Date:  1999-02-19     Completed Date:  1999-02-19     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8400604     Medline TA:  Gen Physiol Biophys     Country:  SLOVAKIA    
Other Details:
Languages:  eng     Pagination:  253-64     Citation Subset:  IM    
Affiliation:
Institute of Experimental Pharmacology, Slovak Academy of Sciences, Bratislava, Slovakia. exfarosa@savba.savba.sk
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MeSH Terms
Descriptor/Qualifier:
Acetylcholine / pharmacology
Animals
Anti-Arrhythmia Agents / pharmacology*
Aorta, Abdominal / drug effects*,  physiology,  physiopathology
Carbolines / pharmacology*
Endothelium, Vascular / drug effects*,  physiopathology,  ultrastructure
Ischemia / physiopathology
Lipid Peroxidation / drug effects,  physiology
Male
Mitochondria / pathology,  ultrastructure
Muscle Contraction / drug effects
Muscle, Smooth, Vascular / drug effects*,  physiology,  physiopathology
Nitroprusside / pharmacology
Norepinephrine / pharmacology
Rats
Rats, Wistar
Reperfusion Injury / prevention & control*
Thiobarbituric Acid Reactive Substances / analysis
Vasoconstriction / drug effects
Vasodilation / drug effects
Chemical
Reg. No./Substance:
0/Anti-Arrhythmia Agents; 0/Carbolines; 0/Thiobarbituric Acid Reactive Substances; 15078-28-1/Nitroprusside; 17411-19-7/dicarbine; 51-41-2/Norepinephrine; 51-84-3/Acetylcholine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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