Document Detail


Endothelial outgrowth and the subsequent cell transition in the culture of embryonic atrioventricular canal placed in an inverted polarity.
MedLine Citation:
PMID:  9663979     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Endothelial cells in the atrioventricular (AV) segment of the developing chicken heart undergo a transition into mesenchymal cells. When the AV segment is explanted onto a hydrated collagen gel, endothelial cells grow out and reproduce in vivo cell transition regardless of the precise orientation of the explant on a gel. Our results showed that when the luminal side of an explant was placed towards a gel surface, the inverted polarity of endothelium was not adjusted by direct reorganization of polarity, but that the endothelium crawled down so as to settle on a gel surface in the correct original cell polarity. Subsequently, endothelial cells showed cytoplasmic hypertrophy, formed microvillous projections and then extended filopodial migratory appendages. These cellular changes were quite similar to those in vivo. However, the continuity of the endothelial layer was specifically disrupted in AV explant cultures. Such disruption was never observed in ventricle explant cultures in which endothelial-mesenchymal cell transition did not occur. The disintegration of AV endothelial outgrowth must be closely related to its capability to transform into mesenchymal cells and mitotic activity to keep a depository of endothelial cells.
Authors:
K Isokawa; J Yi; H Sejima; M Tanaka; K Murakami; H Yokoyama; S Endo; T Komiyama; Y Toda
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of oral science     Volume:  40     ISSN:  1343-4934     ISO Abbreviation:  J Oral Sci     Publication Date:  1998 Mar 
Date Detail:
Created Date:  1998-07-17     Completed Date:  1998-07-17     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9808942     Medline TA:  J Oral Sci     Country:  JAPAN    
Other Details:
Languages:  eng     Pagination:  17-23     Citation Subset:  D; IM    
Affiliation:
Division of Electron Microscopy, Nihon University School of Dentistry, Tokyo, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Culture Techniques
Cell Differentiation
Cell Movement
Cell Polarity
Chick Embryo
Collagen
Endothelium / cytology*
Heart Septum / embryology
Heart Valves / embryology*
Heart Ventricles / embryology*
Mesoderm / cytology*
Myocardium / cytology
Chemical
Reg. No./Substance:
9007-34-5/Collagen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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