| Endothelial nitric oxide gene haplotype reduces the effect of a single bout of exercise on the vascular reactivity in healthy subjects. | |
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MedLine Citation:
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PMID: 22691914 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Polymorphisms in the endothelial nitric oxide synthase (NOS3) gene reduce shear stress-induced nitric oxide production. Thus, we investigated the individual and combined impact of 3 variants in the NOS3 gene (-786T>C, intron 4b4a, and 894G>T) on vascular reactivity before and after exercise. Sedentary, healthy subjects were studied (105 women/26 men, age 32 ± 1 years [mean ± standard error of the mean]). Genotypes were determined by polymerase chain reaction restriction fragment length polymorphism, and haplotypes were determined by a Bayesian-based algorithm. Vascular reactivity was evaluated by the percentage of change in forearm vascular conductance provoked by 5 minutes of circulatory occlusion before (baseline) and 10, 60, and 120 minutes after a maximal cardiopulmonary exercise test. Vascular reactivity increased 10 minutes after exercise in the entire sample (baseline: 218 ± 11 minutes vs 10 minutes: 284% ± 15%, P < .001), remained increased at 60 minutes (239% ± 12%, P = .02 vs baseline), and returned to baseline at 120 minutes (210% ± 10%, P = .83 vs baseline). Genotype analysis showed that subjects with the 894G>T polymorphism had lower vascular reactivity than wild counterparts (group effect, P = .05). Furthermore, subjects with haplotype 2 (H2), containing the -786T>C and 894G>T polymorphisms, had lower vascular reactivity than wild counterparts (haplotype 1 [H1]) (group effect, P = .05), whereas subjects with haplotype 4 (H4), containing only the 894G>T polymorphism, had vascular reactivity similar to that of wild counterparts (H1) (group effect, P = .35). Altogether, these results indicate that the 894G>T polymorphism reduced exercise-mediated increase in vascular reactivity, particularly when it occurred concomitantly with the -786T>C polymorphism. |
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Authors:
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Bruno M Silva; Fabricia J Neves; Natalia G Rocha; Allan R K Sales; Renata F Medeiros; Thales C Barbosa; Felipe S Pereira; Fabiane T Cardoso; Antonio C L Nóbrega |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-6-9 |
Journal Detail:
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Title: Translational research : the journal of laboratory and clinical medicine Volume: - ISSN: 1878-1810 ISO Abbreviation: - Publication Date: 2012 Jun |
Date Detail:
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Created Date: 2012-6-13 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101280339 Medline TA: Transl Res Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2012 Mosby, Inc. All rights reserved. |
Affiliation:
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Laboratory of Exercise Sciences, Department of Physiology and Pharmacology, Fluminense Federal University, Niterói, Rio de Janeiro State, Brazil. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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