Document Detail


Endothelial microparticles: a potential contribution to the thrombotic complications of the antiphospholipid syndrome.
MedLine Citation:
PMID:  15045126     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The antiphospholipid syndrome (APS) refers to persistent anti-phospholipid antibodies (aPL) associated with thrombotic and/or obstetrical complications. The endothelial cell is a target of aPL which can induce a procoagulant and proinflammatory endothelial phenotype, as reported both in vivo and in vitro. Microparticle production is a hallmark of cell activation. In the present study, the presence of endothelial microparticles (EMP) in the plasma of APS patients was investigated. To determine if there is a correlation with certain biological and clinical features, EMP levels were measured in thrombosis-free patients with systemic lupus erythematosus (SLE) patients, with and without aPL, in patients with non aPL-related thrombosis, as well as in healthy controls. Compared to healthy subjects, elevated plasma levels of EMP were found in patients with APS and in SLE patients with aPL, but not in SLE patients without aPL or in non aPL-related thrombosis. EMP levels were also associated with Lupus Anticoagulant (LA) detected by a positive Dilute Russell's Viper Venom time (DRVVT). In parallel, we analyzed the capacity of these plasma to induce vesiculation of cultured endothelial cells. We demonstrated an increase of EMP generated in response to plasma from patients with auto-immune diseases. Interestingly, only APS plasma induced the release of EMP with procoagulant activity. These ex vivo and in vitro observations indicate that generation of EMP in APS and SLE patients results from an autoimmune process involving aPL. Production of procoagulant microparticles in APS patients may represent a new pathogenic mechanism for the thrombotic complications of this disease.
Authors:
Françoise Dignat-George; Laurence Camoin-Jau; Florence Sabatier; Dominique Arnoux; Francine Anfosso; Nathalie Bardin; Véronique Veit; Valéry Combes; Stéphanie Gentile; Valérie Moal; Marielle Sanmarco; José Sampol
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Thrombosis and haemostasis     Volume:  91     ISSN:  0340-6245     ISO Abbreviation:  Thromb. Haemost.     Publication Date:  2004 Apr 
Date Detail:
Created Date:  2004-03-26     Completed Date:  2004-11-19     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7608063     Medline TA:  Thromb Haemost     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  667-73     Citation Subset:  IM    
Affiliation:
INSERM EMI 0019: Physiopathology of the endothelium, UFR de Pharmacie, 27 Bd Jean Moulin, 13385 Marseille Cedex 5, France. dignat@pharmacie.univ-mrs.fr
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MeSH Terms
Descriptor/Qualifier:
Adult
Antiphospholipid Syndrome / complications,  pathology*
Blood Coagulation Tests
Case-Control Studies
Cell Membrane / metabolism*
Cells, Cultured
Endothelial Cells / pathology,  physiology,  ultrastructure
Endothelium, Vascular / pathology,  ultrastructure*
Flow Cytometry
Humans
Lupus Erythematosus, Systemic / blood
Middle Aged
Prospective Studies
Thrombosis / etiology*,  pathology
Comments/Corrections
Comment In:
Thromb Haemost. 2004 Apr;91(4):636-8   [PMID:  15101389 ]

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