Document Detail


Endothelial microparticle release is stimulated in vitro by purified IgG from patients with the antiphospholipid syndrome.
MedLine Citation:
PMID:  23152159     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
IgG antiphospholipid antibodies (aPL) exert direct effects on various cell types, contributing to the pathogenesis of thrombosis and pregnancy morbidity in patients with the antiphospholipid syndrome (APS). Some IgG samples from these patients activate endothelial cells (EC) in vitro as judged by surface expression of adhesion molecules such as E-selectin, which can promote thrombosis. Endothelial microparticles (EMP), which themselves are potentially prothrombotic, are released by activated EC. Though elevated circulating EMP levels have been reported in patients with APS, it is not known whether these EMP are released due to a direct effect of aPL on the cells. We tested the effect of purified polyclonal IgG from patients with APS (APS-IgG) and healthy controls (HC-IgG) upon cultured human umbilical vein EC (HUVEC). HUVEC exposed to APS-IgG produced significantly more EMP than those exposed to HC-IgG (p=0.0036) and a greater proportion of these EMP carried surface E-selectin (6.2% ± 4.0 for APS-IgG vs. 3.4% ± 2.0 for HC IgG, p=0.0172). This study therefore demonstrates that purified polyclonal APS-IgG can drive EMP release. We propose that EMP generation may be a useful measure of aPL-mediated pathogenic effects upon EC.
Authors:
Charis Pericleous; Lindsey A Clarke; Paul A Brogan; David S Latchman; David A Isenberg; Yiannis Ioannou; Ian P Giles; Anisur Rahman
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-11-15
Journal Detail:
Title:  Thrombosis and haemostasis     Volume:  109     ISSN:  0340-6245     ISO Abbreviation:  Thromb. Haemost.     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-01-08     Completed Date:  2013-06-24     Revised Date:  2014-02-20    
Medline Journal Info:
Nlm Unique ID:  7608063     Medline TA:  Thromb Haemost     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  72-8     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Adult
Antibodies, Antiphospholipid / blood*
Antiphospholipid Syndrome / blood,  immunology*
Biological Markers / blood
Case-Control Studies
Cell-Derived Microparticles / immunology*,  metabolism
Cells, Cultured
E-Selectin / metabolism
Female
Human Umbilical Vein Endothelial Cells / immunology*,  metabolism
Humans
Immunoglobulin G / blood*
Middle Aged
Time Factors
Grant Support
ID/Acronym/Agency:
19423//Arthritis Research UK
Chemical
Reg. No./Substance:
0/Antibodies, Antiphospholipid; 0/Biological Markers; 0/E-Selectin; 0/Immunoglobulin G; 0/SELE protein, human

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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