| Endothelial microparticle release is stimulated in vitro by purified IgG from patients with the antiphospholipid syndrome. | |
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MedLine Citation:
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PMID: 23152159 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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IgG antiphospholipid antibodies (aPL) exert direct effects on various cell types, contributing to the pathogenesis of thrombosis and pregnancy morbidity in patients with the antiphospholipid syndrome (APS). Some IgG samples from these patients activate endothelial cells (EC) in vitro as judged by surface expression of adhesion molecules such as E-selectin, which can promote thrombosis. Endothelial microparticles (EMP), which themselves are potentially prothrombotic, are released by activated EC. Though elevated circulating EMP levels have been reported in patients with APS, it is not known whether these EMP are released due to a direct effect of aPL on the cells. We tested the effect of purified polyclonal IgG from patients with APS (APS-IgG) and healthy controls (HC-IgG) upon cultured human umbilical vein EC (HUVEC). HUVEC exposed to APS-IgG produced significantly more EMP than those exposed to HC-IgG (p=0.0036) and a greater proportion of these EMP carried surface E-selectin (6.2% ± 4.0 for APS-IgG vs. 3.4% ± 2.0 for HC IgG, p=0.0172). This study therefore demonstrates that purified polyclonal APS-IgG can drive EMP release. We propose that EMP generation may be a useful measure of aPL-mediated pathogenic effects upon EC. |
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Authors:
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C Pericleous; L A Clarke; P A Brogan; D S Latchman; D A Isenberg; Y Ioannou; I P Giles; A Rahman |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-11-15 |
Journal Detail:
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Title: Thrombosis and haemostasis Volume: 109 ISSN: 0340-6245 ISO Abbreviation: Thromb. Haemost. Publication Date: 2012 Nov |
Date Detail:
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Created Date: 2012-11-15 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7608063 Medline TA: Thromb Haemost Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Charis Pericleous, PhD, Centre for Rheumatology, Division of Medicine University College London, Rayne Institute, 4 th Floor, Room 422, 5 University Street, London, WC1E 6JF, UK, Tel.: +44 2031082158, Fax: +44 2031082152, E-mail: c.pericleous@ucl.ac.uk. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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