| Endothelial evaluation in the cornea bank. | |
MedLine Citation:
|
PMID: 19494636 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The light microscope is the first-choice technique for the evaluation of organ-cultured donor corneas. For the microscopic visualization of the endothelial cells, the corneas have to be immersed in ahypotonic solution. The number of cells, their vitality and morphology are analyzed. The cell densityis easily estimated with the fixed frame/L method. Use of an image analysis system enables computer processing and counting of digitalized endothelial cell images. An adequately high endothelial cell density after the culture period is a decisive criterion that must be met before releasing adonor cornea for grafting. An endothelial cell density of 2,000-2,200 cells/mm2 is generally recognized as the lower limit for the longest possible graft survival. While an evaluation of the endothelium does not necessarily have to be performed at the beginning or middle of organ culture, it isobligatory at the end. Morphology assessment should routinely involve estimating the pleomorphism (deviation from hexagonality), polymegathism (variation in cell area) and granulation/vacuolization of the endothelial cells. Recognition of devitalized cells is easily facilitated by vital stainingwith trypan blue. |
Authors:
|
Jan Schroeter; Peter Rieck |
Related Documents
:
|
16580666 - Corneal integrins and their functions. 3907976 - Assessment of endothelial cell density in bovine corneas after osmotically induced dila... 7960416 - Long-term effects of glaucoma therapy with 4% pilocarpine gel on corneal clarity and en... 3497816 - Specular microscopy of vertebrate corneal endothelium: a comparative study. 19363666 - Role of extracellular signal-regulated kinase for endothelial progenitor cell dysfuncti... 15607886 - Spatial organization of types i and ii collagen in the canine meniscus. |
Publication Detail:
|
Type: Journal Article; Review Date: 2009-06-03 |
Journal Detail:
|
Title: Developments in ophthalmology Volume: 43 ISSN: 0250-3751 ISO Abbreviation: Dev Ophthalmol Publication Date: 2009 |
Date Detail:
|
Created Date: 2009-06-04 Completed Date: 2009-08-14 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8010321 Medline TA: Dev Ophthalmol Country: Switzerland |
Other Details:
|
Languages: eng Pagination: 47-62 Citation Subset: IM |
Copyright Information:
|
Copyright 2009 S. Karger AG, Basel. |
Affiliation:
|
Cornea Bank Berlin, Clinic of Ophthalmology, Charité - Universitatsmedizin Berlin, Campus-Virchow-Klinikum, Berlin, Germany. jan.schroeter@charity.de |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Cell Count Cell Survival Cornea* Corneal Transplantation Endothelium, Corneal / cytology*, physiology Eye Banks / methods*, standards Graft Survival / physiology Humans Organ Culture Techniques Organ Preservation* Quality Control Tissue Donors |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Organ culture preservation for corneal tissue. Technical and quality aspects.
Next Document: Cryopreservation for corneal storage.