Document Detail

Endothelial evaluation in the cornea bank.
MedLine Citation:
PMID:  19494636     Owner:  NLM     Status:  MEDLINE    
The light microscope is the first-choice technique for the evaluation of organ-cultured donor corneas. For the microscopic visualization of the endothelial cells, the corneas have to be immersed in ahypotonic solution. The number of cells, their vitality and morphology are analyzed. The cell densityis easily estimated with the fixed frame/L method. Use of an image analysis system enables computer processing and counting of digitalized endothelial cell images. An adequately high endothelial cell density after the culture period is a decisive criterion that must be met before releasing adonor cornea for grafting. An endothelial cell density of 2,000-2,200 cells/mm2 is generally recognized as the lower limit for the longest possible graft survival. While an evaluation of the endothelium does not necessarily have to be performed at the beginning or middle of organ culture, it isobligatory at the end. Morphology assessment should routinely involve estimating the pleomorphism (deviation from hexagonality), polymegathism (variation in cell area) and granulation/vacuolization of the endothelial cells. Recognition of devitalized cells is easily facilitated by vital stainingwith trypan blue.
Jan Schroeter; Peter Rieck
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Publication Detail:
Type:  Journal Article; Review     Date:  2009-06-03
Journal Detail:
Title:  Developments in ophthalmology     Volume:  43     ISSN:  0250-3751     ISO Abbreviation:  Dev Ophthalmol     Publication Date:  2009  
Date Detail:
Created Date:  2009-06-04     Completed Date:  2009-08-14     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8010321     Medline TA:  Dev Ophthalmol     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  47-62     Citation Subset:  IM    
Copyright Information:
Copyright 2009 S. Karger AG, Basel.
Cornea Bank Berlin, Clinic of Ophthalmology, Charité - Universitatsmedizin Berlin, Campus-Virchow-Klinikum, Berlin, Germany.
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MeSH Terms
Cell Count
Cell Survival
Corneal Transplantation
Endothelium, Corneal / cytology*,  physiology
Eye Banks / methods*,  standards
Graft Survival / physiology
Organ Culture Techniques
Organ Preservation*
Quality Control
Tissue Donors

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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