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Endothelial dysfunction in rat mesenteric artery after regional cardiac ischemia/reperfusion.
MedLine Citation:
PMID:  21948192     Owner:  NLM     Status:  Publisher    
In most previous studies, ischemia-reperfusion (I/R) induced vascular injury referred to injury in the tissue or blood vessel that was actually subjected to I/R. However, less attention has been focused on remote vascular injury that might be caused by cardiac I/R. In the present study, we aimed to assess whether cardiac I/R could affect vasoconstriction and vasodilatation in mesenteric arteries from Sprague-Dawley (SD) rats. Left anterior descending (LAD) coronary arteries from adult male SD rats were occluded (60min) and then reperfused (120min). Changes in hemodynamic parameters indicated that this procedure caused evident cardiac dysfunction. In mesenteric arteries isolated from the animals, cardiac I/R increased significantly the maximum contractions in response to KCl, 5-hydroxytryptamine, phenylephrine, and U46619 and decreased the maximal relaxation to acetylcholine, but not to sodium nitroprusside, compared with sham-operated animals. The nitric oxide synthase inhibitor Nω-nitro-L-arginine methyl (L-NAME) abolished differences of contractile responses to phenylephrine between sham-operated and I/R rats. The anti-oxidant N-acetyl-L-cysteine (NAC) reversed the impairment of acetylcholine vasodilation induced by regional cardiac I/R. However, L-NAME caused a similar degree of inhibition of acetylcholine relaxation in mesenteric arteries from sham-operated and I/R rats. Electron microscopy revealed that mesenteric arterial endothelial structure was degraded in I/R group, and NAC treatment prevented such structural damage. In conclusion, regional cardiac I/R, caused by transient occlusion and reperfusion of the LAD coronary artery, causes peripheral vascular endothelial dysfunction.
Ming Zhao; Xi He; Withrow Gil Wier; Hong-Li Zhang; Mei Zhao; Xiao-Jiang Yu; Wei-Jin Zang
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-9-23
Journal Detail:
Title:  Experimental physiology     Volume:  -     ISSN:  1469-445X     ISO Abbreviation:  -     Publication Date:  2011 Sep 
Date Detail:
Created Date:  2011-9-27     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9002940     Medline TA:  Exp Physiol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
1 Department of Pharmacology, Xi'an Jiaotong University, College of Medicine, Xi'an, 710061, P.R.China;
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