| Endothelial dysfunction following thrombolysis in vitro. | |
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MedLine Citation:
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PMID: 9894489 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVES: Thrombolytic therapy is frequently used to manage vascular graft thrombosis. However, long-term patency after thrombolysis remains poor. The purpose of this study was to characterise the morphological and functional response of endothelial cells (EC) exposed to a thrombus and subsequently lytic therapy. METHODS: Human EC were exposed to human whole blood thrombus for 2, 6, 12, and 24 h. The thrombus was lysed with urokinase. Cell morphology was studied with electron microscopy. Northern blot analyses were performed with human c-DNA probes for endothelin-1, thrombomodulin, tissue factor, tissue plasminogen activator, plasminogen activator inhibitor, and triose phosphate isomerase. RESULTS: EC retraction occurred for each period of incubation. Thrombomodulin expression was increased 2.2-fold at 6 h and 2.4-fold at 24 h. t-PA expression was depressed proportionally to the duration of thrombus exposure. PAI and TF expression transiently increased 1.5-fold at 2 h of exposure and returned to baseline at 6 h. Endothelin expression remained unchanged. CONCLUSIONS: Except for a transient increase in TF expression and reversal of the tPA/PAI ratio, EC exposed to thrombus do not appear to become actively procoagulant. The increase in TM expression may reflect enhanced thromboresistance. However, EC retraction may be responsible for an increase thrombogenicity of saphenous vein graft after thrombosis and Urokinase therapy. |
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Authors:
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A d'Audiffret; P Soloway; R Saadeh; C Carty; P Bush; J J Ricotta; M Dryjski |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: European journal of vascular and endovascular surgery : the official journal of the European Society for Vascular Surgery Volume: 16 ISSN: 1078-5884 ISO Abbreviation: Eur J Vasc Endovasc Surg Publication Date: 1998 Dec |
Date Detail:
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Created Date: 1999-02-03 Completed Date: 1999-02-03 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 9512728 Medline TA: Eur J Vasc Endovasc Surg Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 494-500 Citation Subset: IM |
Affiliation:
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Department of Surgery, SUNY at Buffalo, Millard Fillmore Hospital, NY 14209, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Cells, Cultured Endothelin-1 / analysis Endothelium, Vascular / drug effects, metabolism*, ultrastructure Graft Occlusion, Vascular / drug therapy Humans Microscopy, Electron, Scanning Plasminogen Activator Inhibitor 1 / analysis Saphenous Vein Thrombolytic Therapy / adverse effects* Thrombomodulin / analysis Thromboplastin / analysis Thrombosis / drug therapy Tissue Plasminogen Activator / analysis Urokinase-Type Plasminogen Activator / adverse effects, therapeutic use |
| Chemical | |
Reg. No./Substance:
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0/Endothelin-1; 0/Plasminogen Activator Inhibitor 1; 0/Thrombomodulin; 9035-58-9/Thromboplastin; EC 3.4.21.68/Tissue Plasminogen Activator; EC 3.4.21.73/Urokinase-Type Plasminogen Activator |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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