Document Detail


Endothelial cells promote anti-CD3-induced T-cell proliferation via cell-cell contact mediated by LFA-1 and CD2.
MedLine Citation:
PMID:  7901894     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
T-cell activation requires not only T-cell receptor (TCR) engagement and subsequent TCR/CD3 cross-linking, but also one or more secondary activation signals generated by accessory cells (AC). We investigated the accessory function of endothelial cells (EC) in an in vitro model for T-cell activation where the first cross-linking signal was delivered to peripheral human T lymphocytes by either immobilized anti-CD3 monoclonal antibody (MoAb) or by PHA. In a previous report, we showed that EC provided a potent costimulatory signal in this model system. We have now analysed the nature of the EC-derived costimulatory signal by testing whether EC could be substituted by cytokines, by studying the effect of EC fixation and by testing the involvement of a number of adhesion molecules. Our findings indicate that EC accessory function is mediated mainly by membrane-bound factors. The nature of these membrane-bound factors was analysed by studying the inhibitory properties of a series of MoAbs directed against several adhesion molecules. Antibodies directed against CD44, E-selectin, CD31, CD26, B7/BB1, VLA-4 or VCAM-1 were not inhibitory. However, an inhibition, was clearly observed with antibodies against LFA-1 and CD2. Remarkably, this inhibition was not found with MoAbs to their respective counterstructures ICAM-1 and LFA-3. In summary, we postulate that both LFA-1/ICAM-1, and CD2/LFA-3 interactions are involved in EC accessory function, although the role of the EC-associated adhesion partners is not fully understood.
Authors:
J R Westphal; H W Willems; W J Tax; R A Koene; D J Ruiter; R M De Waal
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Scandinavian journal of immunology     Volume:  38     ISSN:  0300-9475     ISO Abbreviation:  Scand. J. Immunol.     Publication Date:  1993 Nov 
Date Detail:
Created Date:  1993-12-09     Completed Date:  1993-12-09     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0323767     Medline TA:  Scand J Immunol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  435-44     Citation Subset:  IM    
Affiliation:
Department of Pathology, University Hospital Nijmegen, The Netherlands.
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MeSH Terms
Descriptor/Qualifier:
Antibodies, Monoclonal / immunology
Antigen-Presenting Cells / immunology
Antigens, CD2
Antigens, CD3 / immunology*
Antigens, Differentiation, T-Lymphocyte / immunology*
Cell Adhesion Molecules / immunology
Cell Communication
Cells, Cultured
Endothelium, Vascular / cytology,  immunology*
Fluorescent Antibody Technique
Formaldehyde
Humans
Interleukins / immunology
Lymphocyte Activation / immunology*
Lymphocyte Function-Associated Antigen-1 / immunology*
Monocytes / immunology
Phytohemagglutinins
Polymers
Receptors, Immunologic / immunology*
T-Lymphocytes / immunology*
Tissue Fixation
Umbilical Veins
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Antigens, CD2; 0/Antigens, CD3; 0/Antigens, Differentiation, T-Lymphocyte; 0/Cell Adhesion Molecules; 0/Interleukins; 0/Lymphocyte Function-Associated Antigen-1; 0/Phytohemagglutinins; 0/Polymers; 0/Receptors, Immunologic; 30525-89-4/paraform; 50-00-0/Formaldehyde

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