Document Detail


Endothelial cells actively concentrate insulin during its transendothelial transport.
MedLine Citation:
PMID:  23350546     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
We examined insulin's uptake and transendothelial transport (TET) by cultured bovine aortic endothelial cells (BAECs) in order to: a) ascertain whether insulin accumulates within the cells to concentrations greater than in the media; b) compare the TET of insulin to that of inulin (using the latter as a tracer for passive transport or leak); and c) determine whether insulin's TET depended on insulin action. Using (125) I-insulin at physiologic concentrations, we found that BAECs accumulate insulin >5-fold above media concentrations and that the TET of insulin, but not inulin, is saturable and requires intact PI-3-kinase and MEK-kinase signaling. We conclude that the insulin receptor and downstream signaling from the receptor regulate endothelial insulin transport. Based on comparison of the kinetics of BAEC insulin uptake with insulin TET, we suggest that insulin uptake is rate limiting for insulin TET. © 2013 John Wiley & Sons Ltd.
Authors:
Amanda J Genders; Vera Frison; Sarah R Abramson; Eugene J Barrett
Related Documents :
11723056 - Phenotyping of individual pancreatic islets locates genetic defects in stimulus secreti...
3019456 - Comparison of harvesting methods for islet transplantation.
20007976 - Liver x receptor agonists augment human islet function through activation of anapleroti...
765126 - Insulin secretion of isolated islets of sand rats (psammomys obesus) during adaptation ...
14768946 - Cytomegalovirus infection modulates cellular immunity in an experimental model for auto...
18942976 - Ontogenic resistance to powdery mildew in grape berries.
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-28
Journal Detail:
Title:  Microcirculation (New York, N.Y. : 1994)     Volume:  -     ISSN:  1549-8719     ISO Abbreviation:  Microcirculation     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-28     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9434935     Medline TA:  Microcirculation     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2013 John Wiley & Sons Ltd.
Affiliation:
Division of Endocrinology, Department of Medicine, University of Virginia, School of Medicine.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  A c-fos-monomeric red fluorescent protein 1 fusion transgene is differentially expressed in rat fore...
Next Document:  The cytosolic adaptor AP-1A is essential for the trafficking and function of Niemann-Pick type C pro...