Document Detail

Endothelial cells actively concentrate insulin during its transendothelial transport.
MedLine Citation:
PMID:  23350546     Owner:  NLM     Status:  Publisher    
We examined insulin's uptake and transendothelial transport (TET) by cultured bovine aortic endothelial cells (BAECs) in order to: a) ascertain whether insulin accumulates within the cells to concentrations greater than in the media; b) compare the TET of insulin to that of inulin (using the latter as a tracer for passive transport or leak); and c) determine whether insulin's TET depended on insulin action. Using (125) I-insulin at physiologic concentrations, we found that BAECs accumulate insulin >5-fold above media concentrations and that the TET of insulin, but not inulin, is saturable and requires intact PI-3-kinase and MEK-kinase signaling. We conclude that the insulin receptor and downstream signaling from the receptor regulate endothelial insulin transport. Based on comparison of the kinetics of BAEC insulin uptake with insulin TET, we suggest that insulin uptake is rate limiting for insulin TET. © 2013 John Wiley & Sons Ltd.
Amanda J Genders; Vera Frison; Sarah R Abramson; Eugene J Barrett
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-28
Journal Detail:
Title:  Microcirculation (New York, N.Y. : 1994)     Volume:  -     ISSN:  1549-8719     ISO Abbreviation:  Microcirculation     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-28     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9434935     Medline TA:  Microcirculation     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2013 John Wiley & Sons Ltd.
Division of Endocrinology, Department of Medicine, University of Virginia, School of Medicine.
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