Document Detail


Endothelial cells actively concentrate insulin during its transendothelial transport.
MedLine Citation:
PMID:  23350546     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: We examined insulins uptake and transendothelial transport by endothelial cells in order to: (i) ascertain whether insulin accumulates within the cells to concentrations greater than in the media; (ii) compare trans endothelial insulin transport to that of inulin (using the latter as a tracer for passive transport or leaked); and; (iii) determine whether insulins transported depended on insulin action.
METHODS: Using 125I-insulin at physiologic concentrations we measured both the uptake and trans endothelial transport of insulin by bovine aortic endothelial cells and measured cell volume using tritiated 3-O-methylglucose.
RESULTS: Bovine aortic endothelial cells accumulate insulin to > five-fold above the media concentrations and the trans endothelial transport of insulin, but not inulin, is saturable and requires intact PI-3-kinase and MEK signaling.
CONCLUSION: The insulin receptor and downstream signaling from the receptor regulates endothelial insulin transport. Insulin is accumulated against a concentration gradient by the endothelial cell. We suggest that insulin uptake is rate limiting for insulin trans endothelial transport.
Authors:
Amanda J Genders; Vera Frison; Sarah R Abramson; Eugene J Barrett
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Microcirculation (New York, N.Y. : 1994)     Volume:  20     ISSN:  1549-8719     ISO Abbreviation:  Microcirculation     Publication Date:  2013 Jul 
Date Detail:
Created Date:  2013-07-11     Completed Date:  2014-02-04     Revised Date:  2014-07-02    
Medline Journal Info:
Nlm Unique ID:  9434935     Medline TA:  Microcirculation     Country:  United States    
Other Details:
Languages:  eng     Pagination:  434-9     Citation Subset:  IM    
Copyright Information:
© 2013 John Wiley & Sons Ltd.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cattle
Cell Size
Cells, Cultured
Endothelial Cells / cytology,  metabolism*
Insulin / metabolism*,  pharmacology
Mitogen-Activated Protein Kinase Kinases / metabolism
Phosphatidylinositol 3-Kinases / metabolism
Protein Transport / physiology
Grant Support
ID/Acronym/Agency:
DK057878/DK/NIDDK NIH HHS; DK073059/DK/NIDDK NIH HHS; R01 DK057878/DK/NIDDK NIH HHS
Chemical
Reg. No./Substance:
0/Insulin; EC 2.7.1.-/Phosphatidylinositol 3-Kinases; EC 2.7.12.2/Mitogen-Activated Protein Kinase Kinases
Comments/Corrections

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